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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2015, Vol. 35 Issue (6): 40-45    DOI: 10.13523/j.cb.20150607
研究报告     
甲壳素促进茂源链霉菌发酵产酶
刘雅清1, 侯孝仑1, 郭玮婷1, 常忠义1, 高红亮1, 步国健2, 鲁伟2, 解秀娟2, 金明飞1
1. 华东师范大学生命科学学院 上海 200241;
2. 泰兴市东圣食品科技有限公司 泰兴 225411
Enhancement of Transglutaminase Production in Streptoverticillium mobaraensis as Achieved by Treatment with Chitin
LIU Ya-qing1, HOU Xiao-lun1, GUO Wei-ting1, CHANG Zhong-yi1, GAO Hong-liang1, BU Guo-jian2, LU Wei2, XIE Xiu-juan2, JIN Ming-fei1
1. Life Science College of East China Normal University, Shanghai 200241, China;
2. Taixing Dongsheng Food Science and Technology Limited Company, Taixing 225411, China
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摘要：

为了提高茂源链霉菌发酵生产谷氨酰胺转胺酶的产量,研究了甲壳素对茂源链霉菌发酵产酶的影响。结果表明,添加0.5%的甲壳素对茂源链霉菌发酵产酶的促进效果极显著,但甲壳素的添加量达到2%时反而会抑制菌株产酶。从菌株生长代谢过程中pH变化、产酶情况、发酵液中蛋白含量及总氮含量等方面,对甲壳素促进茂源链霉菌发酵产酶的作用机理进行了初步探讨。研究显示,甲壳素在茂源链霉菌发酵过程中对菌体生长产生一定的胁迫,刺激菌体大量分泌次级代谢产物,从而提高茂源链霉菌的产酶。对菌株发酵过程的显微观察则表明,甲壳素也可能通过分散菌体生长,提高菌体向胞外分泌谷氨酰胺转胺酶的量来促进产酶。
关键词： 甲壳素;  茂源链霉菌;  谷氨酰胺转胺酶    
Abstract:

The effects of chitin on the production of transglutaminase (TGase) in Streptoverticillium mobaraensis were tested. It indicated that enzyme activity had increased extremely significantly with the adding of 0.5% chitin compared with control, but 2% of chitin inhibit strains from producing TGase. Next, the mechanism about the enhancement of TGase production by chitin from four aspects,which was the pH changes during the process of metabolism, the production of TGase, the protein content and total nitrogen content in the fermented liquid were discussed. Studies show that chitin produce a certain stress for the growth of strain,thus stimulate the produce of secondary metabolites, so as to improve the production of TGase. Microscopic observation of strain fermentation process shows that it could be improving of the production of TGase by dispersing strains.
Key words: Chitin    Streptoverticillium mobaraensis    Transglutaminase
收稿日期: 2015-03-04 出版日期: 2015-06-25
ZTFLH:  Q935  
基金资助:

行业公益性项目(44400620)资助项目
通讯作者: 金明飞     E-mail: mfjin@bio.ecnu.edu.cn
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引用本文:

刘雅清, 侯孝仑, 郭玮婷, 常忠义, 高红亮, 步国健, 鲁伟, 解秀娟, 金明飞. 甲壳素促进茂源链霉菌发酵产酶[J]. 中国生物工程杂志, 2015, 35(6): 40-45.

LIU Ya-qing, HOU Xiao-lun, GUO Wei-ting, CHANG Zhong-yi, GAO Hong-liang, BU Guo-jian, LU Wei, XIE Xiu-juan, JIN Ming-fei. Enhancement of Transglutaminase Production in Streptoverticillium mobaraensis as Achieved by Treatment with Chitin. China Biotechnology, 2015, 35(6): 40-45.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20150607    或    https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I6/40


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