转录调控因子Rex的功能及调节机制研究进展

doi:10.13523/j.cb.20161013

中国生物工程杂志

2016, Vol. 36

Issue (10): 94-100 DOI: 10.13523/j.cb.20161013

综述

转录调控因子Rex的功能及调节机制研究进展

马力, 吴昊, 王斌斌, 乔建军, 朱宏吉

天津大学化工学院制药工程系系统生物工程教育部重点实验室天津 300072

Progress in Functions and Regulatory Mechanisms of the Transcription Factor Rex

MA Li, WU Hao, WANG Bin-bin, QIAO Jian-jun, ZHU Hong-ji

Key Laboratory of Systems Biotechnology of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

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摘要：

转录因子Rex是一种广泛存在于革兰氏阳性菌，能够与NADH或者NAD⁺直接结合响应胞内NADH/NAD⁺的氧化还原传感器，与靶基因的结合可调节细胞内的多种生理代谢。NAD（H）是调节细胞能量代谢的必需辅酶，显示微生物细胞内的氧化还原状态。研究发现Rex的调节活性与细胞内NADH/NAD⁺比率相关。需氧和厌氧菌属中Rex单体和复合物晶体结构的解析揭示了Rex、NADH/NAD⁺和靶基因间的作用关系及调控机制。通过比较分析了不同菌株中Rex单体和复合物的晶体蛋白结构，并揭示了NADH/NAD⁺对Rex调控活性的影响，进一步解析了Rex与碳和能量代谢、厌氧代谢、发酵、生物膜等之间的联系，并展望了Rex的研究和应用方向。

关键词： Rex; 蛋白结构; NADH/NAD⁺; 转录因子

Abstract:

Transcription factor Rex is a sensor of intracellular NADH/NAD⁺ redox state by incorporating either NADH or NAD⁺ in Gram-positive bacteria. In response to NADH/NAD⁺ ratio, Rex could control many physiological metabolisms by regulating genes expression. NAD[H], the key coenzyme in the metabolic network, could reflect the cellular redox state and influence the activity of Rex. The structures of Rex, either alone or bound with cofactor and/or DNA operator, have been determined in aerobic and anaerobic group. The regulatory mechanism of Rex and the relationship of Rex, NADH/NAD⁺, and target genes were revealed through comparative analysis on its structure in aerobic and anaerobic bacterial. Meanwhile, the connections of Rex with central carbon, energy metabolism, anaerobic metabolism, fermentation, biofilm formation, oxidative stress as well as cell toxicity were elucidated. In addition, further study trends and applications of Rex were discussed.

Key words: NADH/NAD⁺ Rex Protein structure Transcription factor

收稿日期: 2016-03-28 出版日期: 2016-10-25

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通讯作者: 朱宏吉,电子信箱:zhj@tju.edu.cn E-mail: zhj@tju.edu.cn

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引用本文:

马力, 吴昊, 王斌斌, 乔建军, 朱宏吉. 转录调控因子Rex的功能及调节机制研究进展[J]. 中国生物工程杂志, 2016, 36(10): 94-100.

MA Li, WU Hao, WANG Bin-bin, QIAO Jian-jun, ZHU Hong-ji. Progress in Functions and Regulatory Mechanisms of the Transcription Factor Rex. China Biotechnology, 2016, 36(10): 94-100.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20161013 或 https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I10/94

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