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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2018, Vol. 38 Issue (4): 24-29    DOI: 10.13523/j.cb.20180404
研究报告     
中间锦鸡儿CiNAC1基因促进转基因拟南芥叶片的衰老
岳文冉,岳俊燕,张秀娟,杨杞,韩晓东,王瑞刚,李国婧()
内蒙古农业大学生命科学学院 内蒙古自治区植物逆境生理与分子生物学重点实验室 呼和浩特 010018
The CiNAC1 from Caragana intermedia Promotes Transgenic Arabidopsis Leaf Senescence
Wen-ran YUE,Jun-yan YUE,Xiu-juan ZHANG,Qi YANG,Xiao-dong HAN,Rui-gang WANG,Guo-jing LI()
College of Life Sciences, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology, Hohhot 010018, China
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摘要：

NAC转录因子家族是植物特有的、最大的转录因子家族之一,参与植物生物胁迫和非生物胁迫应答、激素信号转导、植物次生生长、细胞分裂和植物衰老等多种过程,在植物生长发育过程中起着重要的作用。以中间锦鸡儿CiNAC1基因的过表达拟南芥纯合体株系为材料,以野生型为对照,对CiNAC1基因功能进行分析。结果发现,乙烯处理后,CiNAC1基因过表达株系与野生型拟南芥相比,叶片衰老提前、叶绿素含量降低、离子渗透率升高。实时荧光定量PCR检测发现,乙烯处理后CiNAC1基因过表达株系中与叶绿素降解相关的基因SGR1、SGR2、PPH,以及与衰老相关的基因SAG13、SAG29、ORE1、SINA1、VNI2和乙烯信号途径中的重要转录因子EIN3的表达量均明显高于野生型拟南芥。表明CiNAC1基因在乙烯诱导的叶片衰老过程中发挥重要作用。
关键词： NAC转录因子;  CiNAC1;  乙烯;  叶片衰老    
Abstract:

NAC transcription factors are one of the largest plant-specific transcription factor families, and play important roles in plant growth and development, such as biotic and abiotic stress response, hormone signaling pathway, plant secondary growth, cell division and plant senescence. The CiNAC1 transgenic Arabidopsis thaliana homozygous lines was compared with wild type for further functional analyzes. The results showed that CiNAC1 overexpression lines promoted leaf senescence after ethylene treatment,furthermore, the chlorophyll contents of leaves were remarkably lower in transgenic lines than in wild-type, and ion leakage increased in transgenic lines. Quantitative real time PCR analysis showed that the expression level of chlorophyll catabolic genes, SGR1, SGR2 and PPH as well as senescence related genes, such as SAG13, SAG29, ORE1, SINA1,VNI2 and EIN3, a key transcription factor in ethylene signaling, was significantly higher in transgenic lines than in wild-type under ethylene treayment. Taken together, these results indicate that CiNAC1 play a role in ethylene induced leaf senescence in A. thaliana.
Key words: NAC transcription factors    CiNAC1    Ethylene    Leaf senescence
收稿日期: 2017-10-26 出版日期: 2018-05-08
ZTFLH:  Q786  
基金资助: 国家自然科学基金(31660065);内蒙古自治区高等学校科学研究项目(NJZY055)
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岳文冉
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杨杞
韩晓东
王瑞刚
李国婧

引用本文:

岳文冉,岳俊燕,张秀娟,杨杞,韩晓东,王瑞刚,李国婧. 中间锦鸡儿CiNAC1基因促进转基因拟南芥叶片的衰老[J]. 中国生物工程杂志, 2018, 38(4): 24-29.

Wen-ran YUE,Jun-yan YUE,Xiu-juan ZHANG,Qi YANG,Xiao-dong HAN,Rui-gang WANG,Guo-jing LI. The CiNAC1 from Caragana intermedia Promotes Transgenic Arabidopsis Leaf Senescence. China Biotechnology, 2018, 38(4): 24-29.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20180404    或    https://manu60.magtech.com.cn/biotech/CN/Y2018/V38/I4/24

图1  EIN3-ORE1-CCGs在调节乙烯介导的叶绿素降解中的通路[16-17]
图2  qRT-PCR检测乙烯处理下CiNAC1基因的表达
图3  野生型和转CiNAC1基因拟南芥株系的衰老表型
图4  乙烯处理诱导野生型和转CiNAC1基因拟南芥叶片衰老
图5  野生型和转CiNAC1基因拟南芥叶绿素含量和离子渗透率的检测
图6  CiNAC1转基因株系中衰老相关基因和叶绿素降解相关基因表达量检测
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