响应面法对小球藻<em>Chlorella zofingiensis</em>高产虾青素条件的优化

中国生物工程杂志

2013, Vol. 33

Issue (7): 71-81

技术与方法

响应面法对小球藻Chlorella zofingiensis高产虾青素条件的优化

王丹, 郑洪立, 纪晓俊, 高振

南京工业大学制药与生物工程学院南京 210009

Optimization the Accumulation of Astaxanthin in Chlorella Zofingiensis Using Response Surface Methodology

WANG Dan, ZHENG Hong-li, JI Xiao-jun, GAO Zhen

College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China

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摘要： 采用析因设计法(Plackett-burman)对影响Chlorella zofingiensis高产虾青素的相关因素进行评价,发现硝酸钠、光照强度、二价铁离子及醋酸钠浓度对虾青素产量影响显著。利用中心组合设计(central composite design)及响应面分析对影响虾青素产量的关键因素做进一步的优化,得到较佳的试验点为二价铁离子浓度0.41 mmol/L, 硝酸钠浓度0.8 mmol/L, 醋酸钠浓度37.1 mmol/L,光照强度650 E/m²×s。优化后虾青素产量从7.890mg/L提高到19.81mg/L, 比优化前提高了2.5倍。

关键词： Chlorella zofingiensis; 虾青素; 优化; 响应面法

Abstract: Statistical experimental designs were used to optimize the accumulation of astaxanthin by Chlorella zofingiensis. First, four most important factors (Fe²⁺, NaNO₃, sodium acetate(NaAc)and light intensity) influencing astaxanthin yield were identified by a two-level Plackett-Burman (PB) design from 9 independent variables. Central Composite Design (CCD) and response surface analysis were adopted to investigate the mutual interactions between these variables and to identify their optimal values that would generate maximum astaxanthin yield. Statistical analysis results showed that interactions between Fe²⁺ and light intensity, NaNO₃ and light intensity affected the astaxanthin yield significantly. The predicted maximum astaxanthin yield (18.88 mg/L) was verified by validation experiments under the optimum conditions of 0.41 mmol/L FeSO₄, 0.8 mmol/L NaNO₃, 37.1 mmol/L NaAc and 650 E/m²×s illumination. Optimal conditions obtained in this experiment resulted in a 2.5-fold increased level of the astaxanthin production prior to initial yield and laid a solid foundation for further use of Chlorella zofingiensis in the production of astaxanthin.

Key words: Chlorella zofingiensis Astaxanthin Optimization Response surface methodology

收稿日期: 2013-02-19 出版日期: 2013-07-25

ZTFLH:

Q815

基金资助: 国家"973"计划项目(2011CB200900);国家自然科学基金(20936002)资助项目

通讯作者: 高振 E-mail: gaozhen@njut.edu.cn

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引用本文:

王丹, 郑洪立, 纪晓俊, 高振. 响应面法对小球藻Chlorella zofingiensis高产虾青素条件的优化[J]. 中国生物工程杂志, 2013, 33(7): 71-81.

WANG Dan, ZHENG Hong-li, JI Xiao-jun, GAO Zhen. Optimization the Accumulation of Astaxanthin in Chlorella Zofingiensis Using Response Surface Methodology. China Biotechnology, 2013, 33(7): 71-81.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I7/71

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