研究报告 |
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气道内微量雾化机对质粒DNA完整性和基因转染效率的影响 |
王虹1,2, 孙文武2, 马壮2 |
1. 第四军医大学西京医院 西安 710032;
2. 沈阳军区总医院全军呼吸和变态反应疾病诊治中心 沈阳 110016 |
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Effect of a New Nebulizer on Plasmid DNA Integrity and Gene Transfection |
WANG Hong1,2, SUN Wen-wu2, MA Zhuang2 |
1. Department of Respiratory Medicine, Xijing Hospital, Fouth Military Medical University, Xi’an 710032, China;
2. The Army Center of Respiratory and Allergic Disease Diagnosis and Management, General Hospital of Shenyang Military Region, Shenyang 110016, China |
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[7] 杨慧瑛, 孙文武, 马壮. 机械喷射式雾化机对质粒DNA完整性的影响及保护性研究. 中国生物工程杂志, 2010, 30(10):44-48. Yang H Y, Sun W W, Ma Z. Effect of Jet Nebulizer on Plasmid DNA Integrity and the Research of Protective Methods. China Biotechnology, 2010, 30(10):44-48.
[8] Lesina E, Dames P, Rudolph C. The effect of CpG motifs on gene expression and clearance kinetics of aerosol administered polyethylenimine (PEI)-plasmid DNA complexes in the lung. J Control Release, 2010, 143(2):243-250.
[9] Jiang H L, Hong S H, Kim Y K, et al. Aerosol delivery of spermine-based poly(amino ester)/Akt1 shRNA complexes for lung cancer gene therapy. Int J Pharm, 2011,420 (2):256-265.
[10] Lentz Y K, Anchordoquy T J, Lengsfeld C S. Rationale for the selection of an aerosol delivery system for gene delivery. J Aerosol Med, 2006, 19(3):372-384.
[11] Sun C, Tang T, Uluda H. Molecular dynamics simulations of PEI mediated DNA aggregation. Biomacromolecules, 2011,12(10):3698-3707.
[12] Goyal R, Tripathi S K, Tyagi S, et al. Linear PEI nanoparticles: efficient pDNA/siRNA carriers in vitro and in vivo. Nanomedicine, 2012,8(2):167-175.
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