木质素是植物细胞一种主要的组成成分，具有重要的生理学功能，它的含量变化将会造成深刻的生态学影响。通过盆栽试验，以不同抗虫转基因棉花及其亲本对照为供试对象，研究不同棉花植株组织中木质素含量及其代谢关键酶活性。结果表明：转Bt基因棉花Z30不同植株组织中木质素含量均比其对照常规棉Z16高，增幅为2.09%~9.45%，但差异未达显著水平；两种转Bt+CpTI棉花不同植株组织中木质素含量均分别比其对照常规棉低，降幅为9.19%~31.95%，其中根系中的木质素含量差异均达到显著水平。与常规棉比较，转基因棉花植株内的苯丙氨酸解氨酶和过氧化物酶活性发生了一些变化，但其显著性改变仅发生在某些品种或某些组织内。对于转基因作物，随着转移基因的多样化，将可能导致其引发的非预期效应愈发不确定与复杂。
As lignin is a major structural component of plant cells, modification in lignin content may have ecological implications. Taking different transgenic cottons as test materials by a pot experiment, the lignin contents, POD and PAL activities in different cotton tissues were studied in this thesis and the changes of lignin metabolism in transgenic cottons were indicated. The results showed that the lignin contents in different plant tissues of transgenic Bt cottons were higher than that in their controls at the degree from 2.09% to 9.45%, and the differences among treatments were not significant. But the lignin contents in different plant tissues of transgenic Bt+CpTI cottons were lower than that in their controls at the degree from 9.19% to 31.95%, and only the difference in roots between transgenic and non-transgenic treatments was significant. In comparing with conventional cottons, POD and PAL activities in transgenic cottons had some changes in certain varieties and tissues. To transgenic crops, unintended effects would be more uncertain and complex along with diversification of transgenic genes.
缪璐,孙彩霞,耿丽. 抗虫转基因棉花苗期木质素含量及其代谢关键酶活性研究*[J]. 中国生物工程杂志, 2006, 26(0): 143-147.
. Studies on Lignin Content and its Key Metabolic Enzymes in Transgenic Cottons. China Biotechnology, 2006, 26(0): 143-147.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I0/143
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