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| Development of an Aptasensor for Electrochemical Detection of Tetracycline |
| CHEN Dan1,2, YAO Dong-sheng1,2, XIE Chun-fang1,3, LIU Da-ling1,3 |
1 Institute of Microbial Technology, Guangzhou 510632, China;
2 National Engineering Research Center of Genetic Medicine, Guangzhou 510632, China;
3 Biological Engineering, Jinan University, Guangzhou 510632, China |
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Abstract The residual antibiotics in agricultural products became one of the most noticeable problems for animal derived food security, which caused many known and potential harm to public health. One of the most common antibiotics used in fodder animals is tetracyclines. Developing the rapid, simple and sensitive biosensor system for tetracyclines detection is very important in food safety control. In this paper, a tetracycline binding aptamer, whose recognition is confirmed by Isothermal Titration Calorimetry, is used as bio-recognizer. The developed biosensor in tetracycline detection and the electrochemical behavior are investigated. Results: By using isothermal titration calorimetry, the aptamer shows a high affinity to tetracycline, the dissociation equilibrium constant is at Kd=51.8μmol/L. According to the differential pulse voltammetry (DPV) analysis, there is a linear relationship between the log concentration of tetracycline and the charge transfer resistance (ΔIp) in the tetracycline conc. ranges from 5.0 to 5.0×103μg/L with correlation coefficient of 0.987 6. The detection limit is at 1.0μg/L within a detection time of 15 min. The detection limit lies obviously lower than the National limited residue of tetracycline (6.0×102μg/L) and also lower than other reported aptasensor for tetracycline.
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Received: 28 August 2013
Published: 25 November 2013
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