|
|
|
| Compare the Effect of NRP-1 Antisense Oligodeoxynucleotides With That of VEGFR-2 Antisense Oligodeoxynucleotides on the Proliferation and Apoptosis of Human Gastric Cancer SGC7901 Cells |
| LI Ben-jie, WU Xiao-ling, WANG Lu-lu |
| Department of Gastrointestinalogy, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China |
|
|
|
Abstract Objective: To investigate and compare the effect of neuropilin-1(NRP-1) antisense oligodeoxynucleotides with that of vascular endothelial growth factor receptor-2 (VEGFR-2) antisense oligodeoxynucleotides on the proliferation and apoptosis of human gastric cancer SGC7901 cells. Methods: Transmit thiophosphorylate modified antisense oligodeoxynucleotides of NRP-1 and VEGFR-2 into human gastric cancer SGC7901 cells with liposome separately and simultaneously, detect NRP-1 mRNA levels and VEGFR-2 mRNA levels by reverse transcriptase-polymerase chain reaction(RT-PCR); Measure the changes of cell proliferation by methyl thiazolyl tetrazolium(MTT); Observe cell apoptosis by flow cytometry. Results: The transcription level of NRP-1 mRNA and VEGFR-2 mRNA in SGC7901 cells after transmission with ASODN decreased. NRP-1 ASODN and VEGFR-2 ASODN inhibit the proliferation of SGC7901 cells and meanwhile promotes the apoptosis of these cells. Such effect intensifies as the concentration of ASODN increases; When NRP-1 ASODN and VEGFR-2 ASODN are used separately, no significant difference is detected. Conclusion: NRP-1 ASODN and VEGFR-2 ASODN inhibited the transcription level as well as the proliferation while they promote the apoptosis level of SGC7901 cells. Compared with that of transmitting ASODNs separately,the effect manifests itself more significantly with the combination of NRP-1 ASODN and VEGFR-2 ASODN.
|
|
Received: 30 November 2011
Published: 25 April 2012
|
|
|
|
|
|
[1] He Z, Tessier-Lavigne M Neuropilin is a receptor for the axonal chemorepellent Semaphorin Ⅲ. Cell,1997,90:739-751.
[2] Ling Wang, Shamit K Dutta. Neuropilin-1 modulates p53/caspases axis to promote endothelial cell survival. PLoS ONE, 2007,2(11): e1161.
[3] Zheng Sujun. The comparison of antisense oligonucleotides、small interfering RNA targeting KDR for gene silencing. Journal of Chongqing Medical University, 2005,30(3):340-344.
[4] Carmeliet P. VEGF as a key mediator of angiogenesis in cancer. Oncology,2005,69 (Suppl 3):4-10.
[5] Geertu Deli, Zhang Mao,Mu Rong,et al.Assessment of the relationship between of VEGF,KDR,and tumor prognosis in colorectal carcinoma and adenoma.2009,3(5):746-755.
[6] Neufeld G,Cohen T, Shraga N,et al.The neuropilins: multifunctional semaphoring and VEGF receptors that modulate axon guidance and angiogenesis. Trends Cardiovasc Med,2002,12(1):13-19.
[7] Hansel D E,Wilentz R E,Yeo C J,et al. Expression of NRP-1 in high grade dysplasis,invasive cancer,and metastases tract.Am J Sung Pathol,2004,28(3):347-356.
[8] Matilde Murga, Oscar Fernandez-Capetillo, Giovanna Tosato. Neuropilin-1 regulates attachment in human endothelial cells independently of vascular endothelial growth factor receptor-2.Blood,2005,105:1992-1999.
[9] Timoshenko A V,Rastogi S,Lala P K.Migration-promoting role of VEGF-C and VEGF-C blinding receptors in human breast cancer cell.Br J Cancer,2007,97(8):1090-1098.
[10] Ren Juan,Dong Lei.Effect of antisense oligodexynucelotide specific to KDR gene on human gastric carcinoma cell.Journal of the Fourth Mititary Medical University, 2002,23(4):333-336.
[11] Hsieh S H,Ying N W,Wu M H,et al.Galectin-1,a novel ligand of neuropilin-1,activates VEGFR-2 signaling and modulates the migration of vascular endothelial cells. Oncogene,2008,64(7):526-531.
[12] Akagi M,Kawaguchi M,Liu W,et al.Induction of neuropilin-1 and vascular endothelial growth factor by epidermalgrowth factor in human gastric cancer cells.Br J Cancer,2003,88(5):796-802.
[13] Ling W,Dutta S K,Kojima T,et al.Neuropin-1 modulates P53/caspases axis to promote endothelial cell surival.PLoS ONE,2007,2(11):e1161.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
