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| Oxygen Glucose Deprivation-induced Apoptosis in Oligodendrocytes Through TRPC3 |
| MENG Zan, XU Dan, LI Zhen, LI Jing, LI Gang, LIU Yong-gang, LIU Zhao yu, WU Hong, TANG Yong, PENG Yan |
| Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China |
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Abstract Objective: To explore whether transient receptor potential canonical 3 (TRPC3) plays a role in the apoptosis of oligodendrocytes induced by oxygen glucose deprivation(OGD).Methods: Control group were established by primary cultured oligodendrocytes of SD rats,model group were established by primary cultured oligodendrocytes of SD rats which exposed to OGD2h,treated group were established by model group+ Pyr3.Western blotting were used to detect the expression of TRPC3 at protein levels.The viability of the oligodendrocytes was determined by MTT assay,and the apoptosis of oligodendrocytes were measured by flow cytometry after AnnexinV-FITC staining,intracellular Ca2+ concentration were measured by flow cytometry and Laser Confocal Scamfing Microscope(LCSM).Results: Specific markers of A2B5 and MBP in oligodendrocytes were positive and the purification is more than 95%,OGD model was established successfully.A significant increase in the expression of TRPC3 in cultured oligodendrocytes.The viability of OGD oligodendrocytes (54.34±6.55)% was obviously decreased and the apoptosis rate was increased (24.24±0.86)% when compared with the control cells (P<0.05).Pyr3 significantly increased the survival rate (72.26±5.41)% and decreased the apoptosis rate (14.82±0.28)% and partially suppressed Ca2+ in oligodendrocytes (P<0.05).Conclusion: The increased expression of TRPC3 which mediated intracellular calcium level may be one of the important reasons for OGD oligodendrocyte apoptosis.
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Received: 05 February 2015
Published: 25 April 2015
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Cite this article:
MENG Zan, XU Dan, LI Zhen, LI Jing, LI Gang, LIU Yong-gang, LIU Zhao yu, WU Hong, TANG Yong, PENG Yan. Oxygen Glucose Deprivation-induced Apoptosis in Oligodendrocytes Through TRPC3. China Biotechnology, 2015, 35(4): 23-29.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150404 OR https://manu60.magtech.com.cn/biotech/Y2015/V35/I4/23
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