Studies on the Effects of Ultrasound on Pepsin,Trypsin and Catalase

China Biotechnology

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Studies on the Effects of Ultrasound on Pepsin,Trypsin and Catalase

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Abstract Three enzymes,pepsin,trypsin and catalase were selected to research the effects and mechanisms of ultrasound on protein.A series of experiments, taking advantage of the change of the biological activity of their solutions under sonication as the index, were done to figure out the main factors influencing the effects of ultrasound on protein. The results indicate that there exist some rules and mechanisms related with the effects of ultrasound on the three enzymes. The three enzymes displayed different mode or degree of activity change under sonication.The degree of the destruction of ultrasound on them rises with the increase of the ultrasound output power and the extension of the time.In addition, the effects of ultrasound on the three enzymes vary with the concentration of their solutions,which means that the degree of the destrcuction of ultrasound could be reduced by adjusting the concentration.The mannitol,a strong free radical scavenger,and Tween-80,a kind of non-ionic surfactant,could protect the activity of the three enzymes under sonication,implying that free radicals produced via the thermal dissociation of water induced by ultrasound cavitation and the cavitation bubbles disturbing the hydrophobic conditions around the enzyme molecules play important roles during the process of the destruction of sonication.However,for different enzyme,different factors dominate the critical role.

Key words： Enzyme activity Pepsin Trypsin Ultrasound

Received: 28 November 2005 Published: 25 May 2006

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. Studies on the Effects of Ultrasound on Pepsin,Trypsin and Catalase. China Biotechnology, 2006, 26(05): 81-84.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I05/81

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