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| LSD1 Regulates the Generation of Induced Pluripotent Stem Cells via Interaction with Oct4/Nanog |
| SUN Hao, LU Cun-fu, GUO Yun-qian |
| College of Biological Sciences and Technology, Center for Computational Biology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 100083, China |
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Abstract Fibroblasts can be reprogrammed to induced pluripotent stem (iPS) cells upon expression of Oct4, Sox2, Klf4 and c-Myc. Transcription factors and chromatin modifiers are important to this reprogramming process. As one of the chromatin regulators, LSD1 plays critical roles in early embryogenesis and ES cell differentiation. Western blot analysis showed that the expression level of LSD1 in ES cells is higher than in MEFs. Overexpression of LSD1 in the reprogramming system has no effect on the generation of iPS cells. However, more iPS cell colonies are formed when the expression of LSD1 is knockdown by RNAi or with LSD1 inhibitor tranylcypromine. Co-immunoprecipitation showed the physical interaction between LSD1 and Oct4/Nanog. In all, these data suggests that LSD1 regulates the generation of iPS cells via interaction with Oct4/Nanog.
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Received: 12 November 2012
Published: 25 December 2012
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