Purification and Antifungal Characterization of a Diketopiperazine from Burkholderia cepacia CF-66

China Biotechnology

2009, Vol. 29

Issue (12): 43-48 DOI:

Purification and Antifungal Characterization of a Diketopiperazine from Burkholderia cepacia CF-66

WANG Ai-jing^1,2,QUAN Chun-shan³,WANG Jian-hua^1,2,WANG Jun-hua³,FAN Sheng-di³

1.Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China
2.Graduate School of Chinese Academy of Sciences,Beijing 100049,China
3.College of Life Science,Dalian Nationalities University,Dalian 116600,China

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Abstract

Fungal diseases cause a considerable damage to many economic crops,while chemical control of diseases has a huge threat to human health and the environment.Therefore,it is becoming extremely urgent to develop biological pesticide with high activity,low toxicity and low residues.Burkholderia cepacia CF-66,isolated from the compost,shows strong antifungal activity due to various secondary metabolites,such as pyrrolnitrin,phenazine,diketopiperazines,siderophores and so on.One of diketoperazines,namely,cyclo(Phe-Pro)(cFP)was purified through the following steps.Firstly the crude extract was obtained from the fermentation broth of B.cepacia CF-66 by centrifugation,reduced pressure distillation and ethyl acetate extraction.Then cFP was isolated and purified by silica gel column chromatography and RP-HPLC.Three peaks were exculsively detected.The third one(cFP)was collected and analyzed by HPLC and GC-MS,showing single peak with a concentration of 15mg/ml.Finally,the antifungal activity was carried out by detecting minimal inhibitory concerntration(MIC),which demonstrated that cFP significantly inhibited the growth of Sclerotinia sclerotiorum,Rhizoctonia solani kühn O-28,Curvularia lunata and so forth.The result of microscopic observation showed that cFP could cause twisted hypha,fractured hypha and swollen tips during hyphal development.In conclusion,cFP has great potential for developing biological pesticide.

Key words： Burkholderia cepacia Diketoperazine Purification Antifungal activity

Received: 03 August 2009 Published: 21 December 2009

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	WANG Ai-Jing
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WANG Ai-Jing, QUAN Chun-Shan, WANG Jian-Hua, WANG Jun-Hua, FAN Ku-Di. Purification and Antifungal Characterization of a Diketopiperazine from Burkholderia cepacia CF-66. China Biotechnology, 2009, 29(12): 43-48.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I12/43


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