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| Optimization of Fermentation Medium of Sucrose Isomerase by Recombinant Escherichia coli through Response Surface Method |
| WANG Chen, LI Sha, XU Hong, WEI Yan, CAI Heng |
| State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology,Nanjing 210009,China |
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Abstract The effects of different concentrations of carbon and nitrogen sources on recombinant E. coli BL21(DE3) on the fermentation of sucrose isomerase were investigated,the culture media of recombinant E.coli producing sucrose isomerase was optimized using Design Expert 7.1.6 software combined with the Plackett-Burman design and central composite design method.The results showed that the optimal media for culture as follows:Cane molasses 10.65 g/L ,Corn steep 22.22 g/L, NaCl 7.57g/L ,MgSO4·7H2O 0.52g/L, KH2PO4 4.46g/L.It is found that the Sucrose isomerase activity was 29.1U/ml, compared to LB medium culture E.coli(15U/ml), sucrose isomerase activity increased by 94%, with the original strain increased 21.4 times(1.3U/ml) compared.
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Received: 03 November 2010
Published: 26 April 2011
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