Study on Preparation of ACE Inhibitory Enzymatic Peptides of Cyanea

doi:10.13523/j.cb.20140315

China Biotechnology

2014, Vol. 34

Issue (3): 103-108 DOI: 10.13523/j.cb.20140315

Study on Preparation of ACE Inhibitory Enzymatic Peptides of Cyanea

JIA Meng-jiao^1,2, LIU Rui^1,2, LU Jie-lin^1,2, ZHU Yun-han^1,2, WANG Ling-chong^1,2, WANG Xin-zhi^1,2, WU Hao^1,2, LU Ming-ming³

1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China;
2. Jiangsu Key Laboratory of Research and Development in Marin Bio-resource Pharmaceutics, Nanjing 210023, China;
3. Jiangsu Nanxing Pharmaceutical Co., Ltd, Nanjing 210023, China

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Abstract Objective: Trypsin was used to prepare ACE inhibitory peptides from Cyanea. Response surface method was used to optimize the ACE inhibitory peptides. The ultratiltration membrane was subjected to obtain ACE inhibitory segments of Cyanea enzymatic hydrolysate. Methods: According to the ACE inhibitiory rate, temperature,reaction time,pH and enzyme dosage were chosen to optimize the hydrolysis conditions,then response surface design was furtherly used to optimize the enzymatic technology.Results: The best conditions were as follows: hydrolysis temperature 49.6℃, enzyme dosage 0.69%, hydrolysis pH 7.8, hydrolysis time 2 hours. The ACE inhibitory rate of hydrolysis peptides at best conditions was 56.79%, compared with the theoretical value of 57.20%, the relative error was 0.41%. The mass segment of Cyanea enzymatic hydrolysate obtained by ultratiltration that molecular weight less than 1kDa had the highest ACE inhibitory activity,which IC₅₀ value is 66.58μg/ml.Conclusion: The preparation of ACE inhibitory enzymatic peptides of Cyanea was determined and its material basis was the mass segment that molecular weight less than 1kDa.

Key words： Cyanea ACE inhibitory peptides Response surface analysis Ultrafiltration enzymatic hydrolysis

Received: 11 November 2013 Published: 25 March 2014

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	JIA Meng-jiao
	LIU Rui
	LU Jie-lin
	ZHU Yun-han
	WANG Ling-chong
	WANG Xin-zhi
	WU Hao
	LU Ming-ming

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JIA Meng-jiao, LIU Rui, LU Jie-lin, ZHU Yun-han, WANG Ling-chong, WANG Xin-zhi, WU Hao, LU Ming-ming. Study on Preparation of ACE Inhibitory Enzymatic Peptides of Cyanea. China Biotechnology, 2014, 34(3): 103-108.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140315 OR https://manu60.magtech.com.cn/biotech/Y2014/V34/I3/103

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