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| Mutagenesis Breeding and Optimization of Cephalosporin C by Cephalosporium acremonium |
| LI Liang, WANG Ze-jian, GUO Mei-jin, CHU Ju, ZHUANG Ying-ping, ZHANG Si-liang |
| State Key Lab of Bioreactor Engineering, National Engineering Research Center for Biotechnology, East China University of Science and Technology, Shanghai 200237, China |
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Abstract The Cephalosporin C fermentation was improved through mutations and optimization of the seed medium. High-producing strains Cephalosporium acremonium FC-1-4 and FC-4-2 were obtained with 60Co irradiation and Uv+LiCl treatment, the production were 26% and 54.5% higher than that of control. Meanwhile, the optimal seed medium component were 2.62% glucose, 3.70% corn steep liquor, 0.15% MgSO4 via Plackett-Burman and central composite design(CCD). The 34.7% and 13.2% increase of Cephalosporin C production was achieved with the Cephalosporium acremonium FC-1-4 and FC-4-2 respectively. The obtained high-producing strains and optimal seed medium have crucial industrial utilization in the future.
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Received: 25 April 2014
Published: 25 August 2014
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