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| Cloning and Function Analysis of the Promoter of PpMADS1 in Peach |
| LI CuiXIE Hua1,2, XU Yong2,LI Yun-fu1,2,MA Rong-cai1,2 |
1.College of Life Science, Capital Normal University, Beijing 100048,China
2.Beijing AgroBiotechnology Research Centre，Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China |
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Abstract In order to analyze transcriptional regulative mechanism of PpMADS1 gene, the PpMADS1 promoter was obtained using Genome Walking method from peach (Prunus persica) genomic DNA. Sequence analysis indicated that there exist TATA-box, CAAT-box, two CArG box, one G-box, one TGA-element and a large number of regulatory elements involved in light response, such as GT-1, Sp1 and as-2-box, these results indicated that the promoter may be regulated by light and hormone. The PpMADS1 promoter was truncated according to the prediction of putative of cis-acting elements and fused with GUS reporter gene and transferred into Arabidopsis thaliana. Histochemical staining of different organs of the transgenic plants showed that the region between -197 to -454bp specified GUS expression in flower primordium and the region between -454 to -678bp in sepals and petals. A negatively regulatory element was shown to be present between -678 to -978bp that repressed GUS expression in filament.
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Received: 20 January 2010
Published: 25 May 2010
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