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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (06): 90-97    DOI: 10.13523/j.cb.20140613
    
Current Status and Prospects of the Expression of Butanol Pathway in Escherichia coli
WANG Qing-long1,2, LIU li2, SHI Ji-ping2, XUE Yong-chang1, SUN Jun-song2
1. School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China;
2. Biorefinery Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
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Abstract  

Biobutanol is regarded as a promising biofuel amid the energy crisis and global problems brought by excess use of fossil energy. In recent years, microorganisms including Escherichia coli are of interests in bioengineering studies for bioproduction of butanol, mostly done by heterologous expression of clostridia derived synthetic butanol pathway. Currently, the maximal yield of butanol production reported in Escherichia coli has been close to that in natural strains; however, there still existed many problems daunting the attempts to further improve the biobutanol level by using this microorganism. The engineered metabolic pathways for formation of n-butanol in E. coli were briefly summarized, the limiting factors existed in bioengineering studies were analyzed, and the possible solutions that might help in further improvement of biobutanol productivity using E. coli as host strain were shared.

Key words： Butanol metabolism      Escherichia coli      Biobutanol     
Received: 12 March 2014      Published: 25 June 2014
ZTFLH:  Q786  
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Cite this article:

WANG Qing-long, LIU li, SHI Ji-ping, XUE Yong-chang, SUN Jun-song. Current Status and Prospects of the Expression of Butanol Pathway in Escherichia coli. China Biotechnology, 2014, 34(06): 90-97.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140613     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I06/90


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