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| Comparison of Three Methods for Detection Newcastle Disease Virus |
| GUO Hao1,2, ZOU Ming-qiang1, YU Dong-sheng3, LIU Xiao-lei2, YUN Cai-lin2, DU Jing-jiao1,4 |
1. Chinese Academy of Inspection and Quarantine, Beijing 100123, China;
2. Inner Mongolia Medical College, Hohhot 010059, China;
3. Inner Mongolia National Center for Mental Health, Hohhot 010010, China;
4. Dalian Medical University, Dalian 116044, China |
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Abstract Newcastle disease viruses (NDV) are detected by three detecting tests and make a comparison of advantages and disadvantages about these tests. The allantoic fluid are obtained through inoculating the NDV virulent F48E9 and avirulent Lasota to SPF chicken embryo and detecting by double antibody sandwich ELISA, suspension array system and RT-PCR. After measure the concentration about antibody 6C4 and 4D9 against NDV, 6C4 was selected as biotin labeled antibody, alternative 4D9 as solid phase capture antibody, the double antibody sandwich detection is based on biotin-streptavidin amplification. Design a group of universal primes against NDV after analyzing the F gene in NDV virulent and avirulent viruses which have been published on genebank and measure the detection limit by RT-PCR. The results show that the detection sensitivity of NDV virulent and avirulent viruses is 1:160 by ELISA, but operation is cumbersome and time-consuming; the detection limit is 1:160 and 1:320 through suspension array system, respectively, however, its operation is more convenient and the equipments are expensive compared with ELISA. The detection limits of RNA in virulent and avirulent viruses are 259pg and 14pg, compared with previous two methods, RT-PCR is a measurement on nucleic acid level, it should have high sensitivity in theory, but the necessary reagents are considerable expensive and the laboratory personnel need training in certain skills.
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Received: 31 May 2011
Published: 25 October 2011
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