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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (03): 81-86    DOI:
    
Microfluidic Chip Application in Stem Cell Research
ZHAO Zhen-li, CAI Shao-xi, DAI Xiao-zhen
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China
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Abstract  

The stem cell has become an ideal supply of cells for tissue engineering and cellular therapies for its’ capacity of long-term self-renewal and multipotency-the ability to differentiate into one or more specialized cell types properties. These approaches require a readily available source of stem cell outside a living body. But the stem cell's microenvironment hasn't been clearly defined for its' multiplicity and complexity, and the conventional culture cell method's limitation also dedicate for it. The stem cell's self-renewal or differentiation fate couldn't be controlled outside a living body with current stem cell research method. As the stem cell is very sensitive for the microenvironment change, precisely mimic and control the stem cell microenvironment that control the stem cell's self-renewal or differentiation fate has become the difficulty in stem cell research. With the introduction of microfluidics into cell culture technology, it is possible that mimic the stem cells’ in vivo microenvironment in vitro. For it can culture stem cell in 3D microenvironment; precisely control the various factors and study their influence on the stem cell fate. Furthermore microfluidic chip can also be made transparent and monitor the stem cells real time by imaging.In the microfluidics and the blood vessel endothelial progenitor cell, review the microenvironment that the stem cell needed and enumerate the advantages of microfluidic technology, summary some successful research findings for the stem cell research inside microfluidics. And the prospect of this technology for the stem cell microenvironment and for other applications was made.



Key wordsStem cells&rsquo      microenvironment      Microfluidic chip      Microtechnology      Soluble factors      Mechanical force      Mimic and regulate in vitro      3-dimensional cell culture     
Received: 08 November 2010      Published: 01 April 2011
ZTFLH:  Q6  
  Q81  
  Q2  
Cite this article:

ZHAO Zhen-li, CAI Shao-xi, DAI Xiao-zhen. Microfluidic Chip Application in Stem Cell Research. China Biotechnology, 2011, 31(03): 81-86.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I03/81

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