Research Progress in Isothermal Amplification of Nucleic Acid Based on Microfluidic Chip

doi:10.13523/j.cb.2011014

China Biotechnology

2021, Vol. 41

Issue (2/3): 116-128 DOI: 10.13523/j.cb.2011014

Research Progress in Isothermal Amplification of Nucleic Acid Based on Microfluidic Chip

SHI Zhong-lin¹,CUI Jun-sheng²,YANG Ke²,HU An-zhong²,LI Ya-nan²,LIU Yong²,DNEG Guo-qing²,ZHU Can-can^2,^**(

),ZHU Ling^2,^**(

)

1 Anhui University, Hefei 230601, China
2 Hefei Institute of Physical Science, Chinese Academy of Sciences,Anhui Institute of Optics and Precision Machinery,Anhui Biomedical Optical Instrument Engineering Technology Research Center,Anhui Medical Optical Diagnosis and Treatment Technology and Equipment Engineering Laboratory,Hefei 230031, China

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Abstract

The isothermal amplification technology of nucleic acid is a molecular amplification technology which can amplify nucleic acid efficiently in a constant temperature system. It can realize exponential growth of target gene in a short time. The microfluidic chip should not be too close to the surface, but should not be too close to the surface. Several steps, such as sample preparation, nucleic acid enrichment, purification and detection, should be integrated onto a “miniaturized”chip, and should be automatically treated to get the experimental result:“sample in, result out”. The combination of the isothermal nucleic acid amplification technology and microfluidic chip can not only realize rapid nucleic acid amplification, but also reduce the dependence on experimental equipment. It has a broad application prospect in bedside instant diagnosis and pathogen rapid screening.The principle of isothermal amplification technology and the application of isothermal amplification technology of nucleic acid based on microfluidic chip are reviewed, and the development trend and application prospect of integrated microfluidic chip are prospeced.

Key words： Microfluidic chip Isothermal nucleic acid amplification Bedside instant diagnosis

Received: 05 November 2020 Published: 08 April 2021

ZTFLH:

Q52Q819

Corresponding Authors: Can-can ZHU,Ling ZHU E-mail: zhucancan@aiofm.ac.cn;zhul@aiofm.ac.cn

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	Zhong-lin SHI
	Jun-sheng CUI
	Ke YANG
	An-zhong HU
	Ya-nan LI
	Yong LIU
	Guo-qing DNEG
	Can-can ZHU
	Ling ZHU

Cite this article:

SHI Zhong-lin,CUI Jun-sheng,YANG Ke,HU An-zhong,LI Ya-nan,LIU Yong,DNEG Guo-qing,ZHU Can-can,ZHU Ling. Research Progress in Isothermal Amplification of Nucleic Acid Based on Microfluidic Chip. China Biotechnology, 2021, 41(2/3): 116-128.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2011014 OR https://manu60.magtech.com.cn/biotech/Y2021/V41/I2/3/116

Table 1 Comparison of performance of several thermostatic amplification techniques

Fig.1 LAMP reaction schematic (a) Designing LAMP primer (b) The forming stage of circulus (c) Cycle amplification stage

Fig.2 Eight-channel PDMS-glass hybrid microfluidic chip for LAMP and Quantitative analysis unit (a) Photograph of an eight-channel PDMS-glass hybrid microfluidic chip (b) Schematic drawing of an eight-channel PDMS-glass hybrid microfluidic chip (c) Photograph of the quantitative analysis unit (d) Schematic illustration of the quantitative analysis unit

Fig.3 RCA reaction schematic

Fig.4 RPA reaction schematic

Fig.5 Photo of foil tray containing liquid reagent container and lyophilized reagent (a)Photograph of the thermoformed lab-on-a-chip cartridge. The foil disc features a chamber with a glass capillary containing 50?L buffer for RPA and a chamber with a centrifuge.A capillary siphon and a centrifuge-pneumatic valve are integrated for fluid control. An aliquoting structure splits the 50?L buffer into 5 × 10μL (b)Photograph of a foil disc assembled with liquid reagent containers and lyophilisate reagents featuring 6 fluidic structures, each capable of processing 5 assays in parallel. For demonstration purposes the buffer is replaced by red ink

Fig.6 HDA reaction schematic

Fig.7 SDA reaction schematic

Fig.8 MDA reaction schematic

Fig.9 Microarray MDA system

Fig.10 NASBA reaction schematic


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