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| Overexpression of A New Evolved Na+/H+ Antiporter Gene AtNHXS1 Improved Salt Tolerance of Tobacco(Nicotiana tabacum L.) |
| HEI Qian, XU Yuan, GUO Li-rong, HUANG Ji-bing, ZHANG Hui, XIA Tao |
| School of Life Science,East China Normal University,Shanghai 200062,China |
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Abstract AtNHXS1 ,a new vacuolar Na+/H+antiporter gene which was obtained from Arabidopsis thaliana AtNHX1 gene by DNA shuffling technology,was transformed into tobacco by Agrobacterium-mediated leaf disc. After hygromycin screened and PCR test,ten transgenic T2 lines were identified,and two lines of which were single gene insertion were used for further salt tolerance assays. Real-time PCR analyses showed that the expression levels of AtNHXS1 mRNA were significantly higher after salt treatment among different tissues,especially in leaves in transgenic lines. After salt stress,the growth status of transgenic lines were dominant better than the wild type lines. Under the treatment of 400 mmol/L NaCl,the transgenic plants grew well but the wild type plants almost nearly died. The result suggested that the transgenic AtNHXS1 tobacco could significantly improve the ability of plant salt tolerance.
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Received: 20 January 2011
Published: 25 August 2011
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