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| Preliminary Research on Gold Nanoparticles Enhancing the Specificity of PCR Amplification of Low Copy Gene from the Complicated System |
| LI Hai-Kuo1, CAO Xue-Yan1, ZHOU Zhi-Lei1, CHEN Heng2 |
1. Nanobiology Laboratory, BioX Life Science Research Center, College of Life Science and Biotechnology,Shanghai JiaoTong University,Shanghai200240,China
2.Shanghai Information Center for Life Sciences|Chinese Academy of Sciences|Shanghai200031,China |
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Abstract Objective：Addition of gold nanoparticles (average diameter10nm) into amplification system with low copy gene fragments from the complicated genome helps to enhance the specificity of PCR amplification. Methods：PCR amplification model system of the complicated genome was simulated, PCR was performed with approximate single copy λ DNA as the template and addition of gold nanoparticles. The experiments were so repeated and optimized that the PCR amplification model system was built by simulating the PCR amplification of the complicated genome with low copy gene. Then, the low copy gene (tumor necrosis factor, TNFα gene exon 1 with 380bp) of human genome was amplified so as to verify the actual efficiency of enhancement of the specificity of PCR amplification by the gold nanoparticles. Results：The gold nanoparticles could better enhance the specificity of PCR amplification in the PCR amplification of the complicated genome system with low copy gene. Conclusion：It was preliminarily indicated that nanoparticle PCR method based on gold nanoparticles could optimize the PCR amplification of the complicated actual genome system with low copy gene, which made an important valuable reference to improvement and extension of the optimized methods of PCR amplification.
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Received: 25 March 2009
Published: 28 July 2009
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