Please wait a minute...

中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2016, Vol. 36 Issue (5): 132-137    DOI: 10.13523/j.cb.20160519
综述     
利用生物工程技术生产蜘蛛丝的研究进展
刘婷婷, 梁梓强, 梁士可, 郭技星, 王方海
中山大学有害生物控制与资源利用国家重点实验室 昆虫学研究所 广州 510275
Research Advances of Producing Spider Silk by Biotechnology
LIU Ting-ting, LIANG Zi-qiang, LIANG Shi-ke, GUO Ji-xing, WANG Fang-hai
State Key Laboratory for Biocontrol/Institute of Entomology, Sun Yat-sen University, Guangzhou 510275, China
 全文: PDF(428 KB)   HTML
摘要:

蜘蛛丝是一种高分子蛋白纤维,具有高强度、高弹性等许多重要的优良特性,在军事、医学、工业、建筑、纺织等领域具有广泛而巨大的应用。然而蜘蛛的产丝量小,且无法高密度养殖以获取大量的蜘蛛丝,难以满足实际应用的需要。于是人们只能着眼于生物工程方法,即将蜘蛛丝蛋白基因转入其它生物体来表达生产蜘蛛丝蛋白,经过多年的研究,已取得很多重要的进展。对蜘蛛丝蛋白在微生物、植物、哺乳动物及家蚕等不同生物载体中表达的研究进展进行重点阐述,并探讨了已有研究的不足和今后研究展望,为进一步探索和研发蜘蛛丝的规模化生产方法提供借鉴与参考。

关键词: 家蚕丝纤维大肠杆菌基因工程动物生物反应器    
Abstract:

Spider silk is a kind of macromolecular protein fiber with high strength, high elasticity, and many other excellent characteristics, so it has been applying widely in military, medicine, industry, construction, textile and other fields. However, spider's silk production quantity is small, and spider can not be high density cultivated for getting a lot of spider silk, it is difficult to meet the needs of practical application. So people can only focus on the biological engineering methods, and try to introduce spider silk protein genes into other organisms to produce spider silk protein. After years of research, a lot of important progress has been made. The research course of spider silk proteins expression in different biological carriers, such as microorganisms, plants, mammals and silkworm were focused, also the deficiency of the existing research and future research prospects were discussed, so as to provide reference for further exploration and development of large-scale production of spider silk.

Key words: Animal bioreactor    Genetic engineering    Silk fiber    Escherichia colio    Silkworm
收稿日期: 2015-12-07 出版日期: 2016-01-04
ZTFLH:  Q81  
基金资助:

广东省科技计划(2014A010107009)资助项目

通讯作者: 王方海     E-mail: lsswfh@mail.sysu.edu.cn
服务  
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章  
王方海
刘婷婷
梁梓强
梁士可
郭技星

引用本文:

刘婷婷, 梁梓强, 梁士可, 郭技星, 王方海. 利用生物工程技术生产蜘蛛丝的研究进展[J]. 中国生物工程杂志, 2016, 36(5): 132-137.

LIU Ting-ting, LIANG Zi-qiang, LIANG Shi-ke, GUO Ji-xing, WANG Fang-hai. Research Advances of Producing Spider Silk by Biotechnology. China Biotechnology, 2016, 36(5): 132-137.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20160519        https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I5/132

[1] 潘志娟, 李春萍, 盛家镛. 高性能蛋白质纤维蜘蛛丝的研究与应用(1). 丝绸, 2004, 10: 40-43. Pan Z J, Li C P, Sheng J Y. The research and application of high-performance spider silk protein fiber (1). Silk, 2004, 10: 40-43.
[2] 赵爱春, 夏庆友, 鲁成, 等. 超级纤维蜘蛛丝的研究动向. 蚕学通讯, 2007, 27(2):28-34. Z hao A C, Xia Q Y, Lu C, et al. Research trend in super fiber spider silks. News letter of Sericultural Science, 2007, 27(2):28-34.
[3] 王琳纲,胡平. 蜘蛛丝的研究进展及展望. 新材料产业, 2007, 9:63-67. Wang L G, Hu P. The research progress of spider silk and prospects. Advanced Materials Industry, 2007, 9: 63-67.
[4] Lewis R V, Hinman M, Kothakota S, et al. Expression and purification of a spider silk protein: a new strategy for producing repetitive proteins. Protein Expression and Purification, 1996, 7(4): 400-406.
[5] Scheller J, Gührs K H, Grosse F, et al. Production of spider silk proteins in tobacco and potato.Nature Biotechnology, 2001, 19(6): 573-577.
[6] Lazaris A, Arcidiacono S,Huang Y,et al. Spider silk fiber spun from soluble recombinant silk produced in mammalian cells.Science, 2002, 295(5554) : 472- 476.
[7] 张袁松,赵天福,赵爱春,等. 转基因家蚕生产含蜘蛛丝蛋白的新型复合茧丝纤维. 纺织学报,2012, 33(5): 1-5. Zhang Y S, Zhao T F, Zhao A C, et al. New silk fiber composite containing spider dragline silk protein and produced by transgenic silkworm. Journal of Textile Research, 2012, 33(5): 1-5.
[8] Prince J T,Mcgrath K P,Di Girolamo C M,et al. Construction,cloning and expression of synthetic genes encoding spider dragline silk. Biochemistry, 1995, 34(34): 10879-10885.
[9] Xia X X, Qian Z G, Ki C S, et al. Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber. Proc Natl Acad Sci USA, 2010, 107(32): 14059-14063.
[10] Fahnestock S R, Irwin S L. Synthetic spider dragline silk proteins and their production in Escherichia coli. Applied Microbiology and Biotechnology, 1997, 47(1): 23-32.
[11] Fahnestock S R, Bedzyk L A. Production of synthetic spider dragline silk protein in Pichia pastoris. Applied Microbiology and Biotechnology, 1997, 47(1): 33-39.
[12] 杜文华,赵天福,朱勇. 蜘蛛丝蛋白基因工程的研究进展. 蚕业科学, 2011, 37(5): 892-898. Du W H, Zhao T F, Zhu Y. Advances in genetic engineering of spider silk proteins. Science of Sericulture, 2011, 37(5): 892-898.
[13] Scheller J, Henggeler D, Viviani A, et al. Purification of spider silk-elastin from transgenic plants and application for human chondrocyte proliferation.Transgenic Res, 2004, 13(1): 51-57.
[14] Barr L A, Fahnestock S R, Yang J J, et al. Production and purification of recombinant DP1B silk-like protein in plants. Mol Breeding, 2004, 13(4): 345- 356.
[15] Piruzian E S, Bogush V G, Sidoruk K V, et al. Construction of syn-thetic genes for analogs of spider silk spidroin 1 and their expression in tobacco plants. Mol Biol, 2003, 37(4): 554-560.
[16] Yang J J, Barr L A, Fahnestock S R, et al. High yield recombinant silk-like protein production in transgenic plants through protein targeting. Transgenic Res, 2005, 14(3): 313-324.
[17] Patel J, Zhu H, Menassa R, et al. Elastin-like polypeptide fusions enhance the accumulation of recombinant proteins in tobacco leaves. Transgenic Res, 2007, 16(2): 239-249.
[18] Hauptmann V, Weichert N, Rakhimova M, et al. Spider silks from plants - a challenge to create native-sized spidroins. Biotechnology Journal, 2013, 8(10): 1183-1192.
[19] Xu H T, Fan B L,Yu S Y, et al. Construct synthetic gene encoding artificial spider dragline silk protein and its expression in milk of transgenic mice.Anim Biotechnol, 2007, 18:1-12.
[20] 段亚峰, 冀勇斌. 蜘蛛丝开发应用的现状与进展. 丝绸, 2002, 7: 46-47. Duan Y F, Ji Y B. The situation and progress of the exploitation and application of spider silk. Silk, 2002, 7:46-47.
[21] 张慧勤, 王志新. 蜘蛛丝的研究与应用. 中原工学院学报, 2005, 16(4): 49-50. Zhang H Q, Wang Z X. Research and application of spider silk. Journal of Zhongyuan Institute of Technology, 2005, 16(4): 49-50.
[22] 杨华军, 王丹, 李兴华. 蜘蛛丝的基础和应用研究概况. 蚕桑通报, 2009, 40(3): 2-4. Yang H J, Wang D, Li X H. Research advances on spider silk and its application. Bulletin of Sericulture, 2009, 40(3): 2-4.
[23] Williams D.Sows' ears, silk purses and goats' milk: new production methods and medical applications for silk.Med Device Technol, 2003, 14(5): 9-11.
[24] Tokareva O, Valquiria A, Lacerda M, et al. Recombinant DNA production of spider silk proteins. Microbial Biotechnology, 2013, 6(6): 651-663.
[25] Miao Y G, Zhang Y S, Nakagaki K, et al. Expression of spider flagelliform silk protein in Bombyx mori cell line by a novel Bac-to-Bac/BmNPV baculovirus expression system. Applied Microbiology and Biotechnology, 2006, 71(2): 192-197.
[26] Zhang Y S, Hu J H, Miao Y G, et al. Expression of EGFP-spider dragline silk fusion protein in Bm N cells and larvae of silkworm showed the solubility is primary limit for dragline proteins yield. Mol Biol Rep, 2008, 35(3): 329- 335.
[27] Yamao M, Katayama N, Nakazawa H, et al. Gene targeting in the silkworm by use of a baculovirus. Gene Dev, 1999, 13(5): 511-516.
[28] Tamura T, Thibert C, Royer C, et al. Germline transformation of the silkworm Bombyx mori L. using a piggybac transposon-derived vector. Nat Biotechnol, 2000, 18(1): 81-84.
[29] 郑青亮, 蒋彩英, 张耀洲. 蜘蛛丝的结构性能及表达策略研究进展. 蚕业科学, 2009, 35(3): 685-691. Zheng Q L, Jiang C Y, Zhang Y Z. Progress of studies on the structure and properties of spider silk and its expression strategies. Science of Sericulture, 2009, 35(3): 685-691.
[30] Wen H X, Lan X Q, Zhang Y S, et al. Transgenic silkworms (Bombyx mori) produce recombinant spider dragline silk in cocoons.Mol Biol Rep, 2010, 37(4):1815-1821.
[31] Teule F, Miao Y G, Sohn B H, et al. Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties.Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(3):923-928.
[32] Kuwana Y, Sezutsu H, Nakajima K, et al. High-toughness silk produced by a transgenic silkworm expressing spider (Araneus ventricosus) dragline silk protein. PLoS ONE, 2014, 9(8): e105325.

[1] 乔圣泰,王曼琦,徐慧妮. 番茄SlTpx原核表达蛋白的体外功能分析*[J]. 中国生物工程杂志, 2021, 41(8): 25-32.
[2] 何若昱,林福玉,高向东,刘金毅. 信号肽在大肠杆菌分泌系统中的研究与应用进展[J]. 中国生物工程杂志, 2021, 41(5): 87-93.
[3] 吴弘轩, 杨金花, 沈培杰, 李清晨, 黄建忠, 祁峰. 利用大肠杆菌细胞工厂生产吲哚-3-乙酸的研究 *[J]. 中国生物工程杂志, 2021, 41(1): 12-19.
[4] 闫伟欢,黄统,洪解放,马媛媛. 丁醇在大肠杆菌中的生物合成研究进展*[J]. 中国生物工程杂志, 2020, 40(9): 69-76.
[5] 彭向雷,王烨,王丽男,苏彦斌,付远辉,郑妍鹏,何金生. 单引物PCR法引入定点突变 *[J]. 中国生物工程杂志, 2020, 40(8): 19-23.
[6] 童梅,程永庆,刘金毅,徐晨. 促进大肠杆菌周质空间小分子抗体表达的菌种构建方法*[J]. 中国生物工程杂志, 2020, 40(5): 48-56.
[7] 杨丽,石晓宇,李文蕾,李剑,徐寒梅. 构建噬菌体展示抗体库过程中电穿孔法的条件优化[J]. 中国生物工程杂志, 2020, 40(4): 42-48.
[8] 刘迪,张洪春. 慢性阻塞性肺疾病基因工程动物模型研究进展 *[J]. 中国生物工程杂志, 2020, 40(4): 59-68.
[9] 乐易林,傅毓,倪黎,孙建中. 热稳定性丙酮酸:铁氧还蛋白氧化还原酶异源表达及其在乙酰辅酶A合成中的应用 *[J]. 中国生物工程杂志, 2020, 40(3): 72-78.
[10] 陈春琳,秦松,宋宛霖,刘志丹,刘正一. 褐藻寡糖生物法制备研究进展 *[J]. 中国生物工程杂志, 2020, 40(10): 85-95.
[11] 杭海英,刘春春,任丹丹. 流式细胞术的发展、应用及前景 *[J]. 中国生物工程杂志, 2019, 39(9): 68-83.
[12] 赵程程,孙长坡,常晓娇,伍松陵,林振泉. 大肠杆菌细胞裂解系统的构建及其在真菌毒素降解酶表达中的应用 *[J]. 中国生物工程杂志, 2019, 39(4): 69-77.
[13] 马淑霞,张玲,闫晋飞,游松. 裂壶藻脂肪酸合酶途径合成多不饱和脂肪酸的研究 *[J]. 中国生物工程杂志, 2018, 38(9): 27-34.
[14] 贺雪婷,张敏华,洪解放,马媛媛. 大肠杆菌丁醇耐受机制及耐受菌选育研究进展 *[J]. 中国生物工程杂志, 2018, 38(9): 81-87.
[15] 王嘉祯,姚伦广,王峰,阚云超,罗金萍,黄倩倩,段建平. 家蚕中肠特异启动子P56的克隆及活性分析 *[J]. 中国生物工程杂志, 2018, 38(2): 13-17.