利用统计学实验设计（RSM）对能够利用甲烷的假单胞菌菌株ME16主要培养条件进行了优化。以液体无机盐和甲烷气体作为培养基分别进行了温度、接种量、甲烷含量和培养pH对细菌生长影响的研究，并在此基础上，利用响应面法分析优化了ME16菌株的主要培养条件，得到最佳培养条件为：温度29.4℃，接种量1.8%，甲烷含量25%。采用优化培养条件进行培养，细菌生物量增大0.8倍，达到稳定期的培养时间缩短了50h。该菌株初步应用于甲烷气体的脱除，脱除率达65.7 %，表明该菌株能良好的脱除空气中甲烷。
Statistic-based experimental design (RSM) was used to optimize the culture conditions for Pseudomonas aeruginosa ME16 strain which capable of utilizing methane. Effects of inoculum volume, temperature, methane content, and initial pH of media on cell growth were studied with the medium of mineral salt and methane gas, then the response surface analysis was used to optimize culture conditions, The optimum conditions found are: temperature 29.4℃, inoculum volume 1.8% and methane content 25%. The optimized culture conditions allowed the production to be increased 0.8 times and cultivation time to be decreased. The removal of methane gas was preliminary studied by ME16 , the removal rate for methane gas reached 65.7%. The results indicated that the strain capable of removing of methane.
郑军,程红兵,刘尚俊,赵艮贵. 甲烷利用菌培养条件的优化及其初步应用[J]. 中国生物工程杂志, 2007, 27(12): 80-83.
. Optimization of Culture conditions for a methane utilizing strain and its preliminary application. China Biotechnology, 2007, 27(12): 80-83.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2007/V27/I12/80
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