头孢菌素C产生菌的诱变育种及培养基优化

doi:10.13523/j.cb.20140810

中国生物工程杂志

2014, Vol. 34

Issue (8): 61-66 DOI: 10.13523/j.cb.20140810

技术与方法

头孢菌素C产生菌的诱变育种及培养基优化

黎亮, 王泽建, 郭美锦, 储炬, 庄英萍, 张嗣良

华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心上海 200237

Mutagenesis Breeding and Optimization of Cephalosporin C by Cephalosporium acremonium

LI Liang, WANG Ze-jian, GUO Mei-jin, CHU Ju, ZHUANG Ying-ping, ZHANG Si-liang

State Key Lab of Bioreactor Engineering, National Engineering Research Center for Biotechnology, East China University of Science and Technology, Shanghai 200237, China

全文: PDF(773 KB) HTML

摘要：

通过对顶头孢霉（Cephalosporium acremonium）FC-01进行诱变选育及特定种子培养基的优化，提高了头孢菌素C的发酵产量。分别采用紫外-氯化锂和钴-60（60Co）γ射线对FC-01进行诱变选育，筛选到高产菌株FC-1-4和FC-4-2，产量较出发菌株分别提高了26%和54.5%。运用Plackett-Burman设计方法和响应面法对种子培养基进行优化，头孢菌素C发酵效价较对照分别提高了34.7%和13.2%，优化后的种子培养基主要成分为玉米浆3.70%、葡萄糖2.62%和硫酸镁0.15%，得到的菌株及相应的种子培养条件已成功应用在160M³工业发酵罐生产中，具有重要的工业生产能力。

关键词： 头孢菌素C; 诱变育种; 响应面法; 发酵

Abstract:

The Cephalosporin C fermentation was improved through mutations and optimization of the seed medium. High-producing strains Cephalosporium acremonium FC-1-4 and FC-4-2 were obtained with ⁶⁰Co irradiation and Uv+LiCl treatment, the production were 26% and 54.5% higher than that of control. Meanwhile, the optimal seed medium component were 2.62% glucose, 3.70% corn steep liquor, 0.15% MgSO₄ via Plackett-Burman and central composite design(CCD). The 34.7% and 13.2% increase of Cephalosporin C production was achieved with the Cephalosporium acremonium FC-1-4 and FC-4-2 respectively. The obtained high-producing strains and optimal seed medium have crucial industrial utilization in the future.

Key words: Cephalosporin C Mutagenesis breeding Response surface methodology Fermentation

收稿日期: 2014-04-25 出版日期: 2014-08-25

ZTFLH:

Q345

基金资助:

高等学校博士学科点专项科研基金资助项目（20110074110015）

通讯作者: 储炬，E-mail：juchu@ecust.edu.cn E-mail: juchu@ecust.edu.cn

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引用本文:

黎亮, 王泽建, 郭美锦, 储炬, 庄英萍, 张嗣良. 头孢菌素C产生菌的诱变育种及培养基优化[J]. 中国生物工程杂志, 2014, 34(8): 61-66.

LI Liang, WANG Ze-jian, GUO Mei-jin, CHU Ju, ZHUANG Ying-ping, ZHANG Si-liang. Mutagenesis Breeding and Optimization of Cephalosporin C by Cephalosporium acremonium. China Biotechnology, 2014, 34(8): 61-66.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140810 或 https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I8/61

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