PLGA支架降解对血管化功能影响的体外研究

中国生物工程杂志

2012, Vol. 32

Issue (07): 16-19

研究报告

PLGA支架降解对血管化功能影响的体外研究

武延格, 孙学峰, 杨林, 王正

暨南大学第二临床医学院深圳市人民医院深圳 518020

Effects of PLGA Degradation on Angiogenesis in vitro

WU Yan-ge, SUN Xue-feng, YANG Lin, WANG Zheng

Shenzhen People's Hospital, the Second Clinical Medical College of Jinan University, Shenzhen 518020, China

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摘要： 目的:研究聚乳酸-羟基乙酸 (PLGA) 支架材料降解产物对血管内皮细胞增殖、迁移和小管样结构形成的影响。方法:将PLGA支架材料放入磷酸盐缓冲液 (PBS)中体外无菌降解1、2、4周。用降解液处理人脐静脉内皮细胞株HUVEC,采用Brdu ELISA法、Transwell小室法和小管形成实验检测PLGA支架材料降解液对血管内皮细胞增殖、迁移和小管样结构形成的影响。结果:PLGA支架材料1周降解液对内皮细胞的迁移和小管形成无明显影响,对内皮细胞增殖有一定的促进作用。随着降解时间的延长,2周降解液抑制内皮细胞的迁移和小管形成,4周的降解液对内皮细胞增殖、迁移和小管形成均有抑制作用。结论:PLGA支架材料降解初期有对血管内皮细胞的增殖有促进作用,降解后期可能由于降解过程中产生的酸性物质累积增多,影响了血管内皮细胞的生长和功能,从而抑制新生血管的形成。

关键词： PLGA; 降解; 血管化功能

Abstract: Objective: To investigate the influences of degradable PLGA scaffold on proliferation, migration and the formation of tube-like structure (TLS formation) of vascular endothelial cells.Methods: PLGA scaffold were immersed in PBS fluid to stimulate the degradation for 1, 2, and 4 weeks. HUVEC (Human Umbilical Vein Endothelial Cells) was cultured with degradation fluid. Cell proliferation, migration and TLS formation were detected by Brdu ELISA, Transwell chamber and tube formation.Results: The PLGA degradation fluid showed no effects on the migration, TLS formation of HUVEC, but the proliferation was increased in 1 week. With the prolonged degradation time, the migration and TLS formation were significantly decreased in 2 weeks, and the proliferation, migration and TLS formation were reduced remarkably in 4 weeks.Conclusions: At the beginning of degradation, PLGA could improve the proliferation function. The concentration of acid products increased with the increase of degradation time, and cell behaviors were retrained, thus inhibiting the angiogenesis of endothelial cells.

Key words: PLGA Degradation Angiogenic function

收稿日期: 2012-02-01 出版日期: 2012-07-25

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通讯作者: 杨林 E-mail: yanglin70@yahoo.com

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引用本文:

武延格, 孙学峰, 杨林, 王正. PLGA支架降解对血管化功能影响的体外研究[J]. 中国生物工程杂志, 2012, 32(07): 16-19.

WU Yan-ge, SUN Xue-feng, YANG Lin, WANG Zheng. Effects of PLGA Degradation on Angiogenesis in vitro. China Biotechnology, 2012, 32(07): 16-19.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I07/16

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