诱导回输胰岛素分泌细胞对大鼠糖尿病的疗效

中国生物工程杂志

2011, Vol. 31

Issue (5): 94-98

技术与方法

诱导回输胰岛素分泌细胞对大鼠糖尿病的疗效

袁凤山¹, 王长军², 董飞¹, 赵宇航¹

1. 中国医科大学附属第一医院沈阳 110001;
2. 辽宁省铁岭市卫生局铁岭 112000

The Therapeutic Effect of the Induced Insulin Secreting Cells on Rat Diabetes

YUAN Feng-shan¹, WANG Chang-jun², DONG Fei¹, ZHAO Yu-hang¹

1. The First Affiliated Hospital, China Medical University, Shenyang 110001,China;
2. Health Bureau of Tieling City, Liaoning Province,Tieling 112000,China

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摘要：

目的:研究胰岛素分泌细胞的体外诱导方法及其对大鼠糖尿病的疗效。方法:分离培养大鼠骨髓干细胞,用尼克酰胺及肠促胰岛素类似物诱导其分化为胰岛素分泌细胞。将24只Wistar大鼠随机分为对照组、糖尿病组和诱导组。后两组建立糖尿病模型,将该胰岛素分泌细胞回输至诱导组体内,监测大鼠体重、血糖(空腹及OGTT 120分血糖)及空腹胰岛素等。实验终止时取大鼠肝脏、脾脏、胰腺,检测胰岛素和BrdU染色双阳性细胞。结果:治疗后诱导组大鼠胰岛素水平显著高于糖尿病组(P<0.05),空腹及OGTT 120分血糖均低于糖尿病组( P均<0.05)。诱导组胰腺中胰岛素阳性细胞百分比明显高于糖尿病组(P<0.05),诱导组的胰腺、肝脏及脾脏中发现少量胰岛素和BrdU双阳性细胞。结论:尼克酰胺联合肠促胰岛素类似物可诱导骨髓干细胞分化为胰岛素分泌细胞;该胰岛素分泌细胞能提高胰岛素水平,降低血糖,对大鼠糖尿病有明显治疗作用。

关键词： 糖尿病; 骨髓干细胞; 胰岛素分泌细胞

Abstract:

Objective: To observe the induction of differentiation and therapeutic effect of insulin secreting cells on rat diabetes. Methods: The bone marrow stem cells were extracted form rats and induced into insulin secreting cells with nicotinamide and exendin-4. 24 Wistar rats were randomized into normal, diabetes, and induction group. The diabetes model were established for the diabetes group and induction group. The induced cells were intraperitoneally injected into rats of the induction group. The weight, blood glucose ( fasting and OGTT 120min ), and fasting serum insulin were measured. At the end of the experiment, liver, spleen, pancreas were removed, and the double positive(insulin and BrdU) cells were calculated by immunohistochemistry. Results: At the end of administration, the fasting serum insulin of induction group was significant higher than the diabetes group (P<0.05), and the fasting and OGTT 120min blood glucose was lower than the diabetes group(P both<0.05). The ratio of insulin positive cells in the induction group was higher than diabetes group, and some insulin and BrdU duble-positive cells were found in pancreas, liver, and spleen of induction group. Conclusion: Nicotinamide and exendin-4 can induce bone marrow stem cells into insulin secreting cells, and these cells can increase the serum insulin, reduce the serum glucose, then improve the rat diabetes.

Key words: Diabetes mellitus Bone marrow stem cells Insulin-secreting cells

收稿日期: 2010-11-02 出版日期: 2011-05-27

ZTFLH:

R456

基金资助:

辽宁省教育厅高等学校科学研究资助项目(05L573)

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引用本文:

袁凤山, 王长军, 董飞, 赵宇航. 诱导回输胰岛素分泌细胞对大鼠糖尿病的疗效[J]. 中国生物工程杂志, 2011, 31(5): 94-98.

YUAN Feng-shan, WANG Chang-jun, DONG Fei, ZHAO Yu-hang. The Therapeutic Effect of the Induced Insulin Secreting Cells on Rat Diabetes. China Biotechnology, 2011, 31(5): 94-98.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I5/94

[1] Madec A M, Mallone R, Afonso G, et al. Mesenchymal stem cells protect NOD mice from diabetes by inducing regulatory T cells. Diabetologia, 2009,52(7):1391-1399.

[2] 孙晓晖,王颜刚.干细胞移植定向分化为胰岛β细胞治疗糖尿病.中国组织工程研究与临床康复,2009,13(1):165-168. Sun X H, Wang Y G. Journal of Clinical Rehabilitative Tissue Engineering Research, 2009,13(1):165-168.

[3] 赵春华.干细胞原理、技术与临床应用.北京:化学工业出版社, 2006, 63-81. Zhao C H. Principle, Technology, and Clinic Application of Embryonic Cells. Beijing: Chemical Industry Press, 2006, 63-81.

[4] Choi K S, Shin J S, Lee J J, et al. In vitro trans-differentiation of rat mesenchymal cells into insulin-producing cells by rat pancreatic extract. Biochem Biophys Res Commun, 2005,330(4): 1299-1305.

[5] Da Silva M L, Chagastelles P C, Nardi N B. Mesenchymal stem cells reside in virtually all post-natal organs and tissues. J Cell Sci, 2006,119(Pt 11):2204-2213.

[6] 刘伯轩,刘师伟,王建华,等.骨髓间充质干细胞移植后2型糖尿病鼠血糖及肾脏病变的改善.中国组织工程研究与临床康复,2009,13(10):1872-1876. Liu B X, Liu S W, Wang J H, et al. Journal of Clinical Rehabilitative Tissue Engineering Research, 2009,13(10):1872-1876.

[7] Tropel P, Nale D, Platet N. Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Exp Cell Res, 2004,295(2):395-406.

[8] Bai L, Meredith G, Tuch B E. Glucagon-like peptide-1 enhances production of insulin in insulin-producing cells derived from mouse embryonic stem cells. J Endocrinol, 2005, 186(2): 343-352.

[9] Guerci B, Martin C S. Exenatide: its position in the treatment of type 2 diabetes.Ann Endocrinol, 2008,69(3):201-209.

[10] Gao F, Wu D Q, Hu Y H, et al. In vitro cultivation of islet-like cell clusters from human umbilical cord blood-derived mesenchymal stem cells. Transl Res, 2008,151(6):293-302.

[11] Yu B S, Wang A R. Glucagon-like peptide 1 based therapy for type 2 diabetes. World J Pediatr, 2008,4(1): 8-13.

[12] Gao F, Wu D Q, Hu Y H, et al. In vitro cultivation of islet-like cell clusters from human umbilical cord blood-derived mesenchymal stem cells. Transl Res, 2008, 151(6):293-302.

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