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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2023, Vol. 43 Issue (7): 101-113    DOI: 10.13523/j.cb.2301008
    
Research Progress of Electrochemical Biosensor in the Detection of Tumor Markers in Breast Cancer
Jia-ye JIANG1,Ya-fang WU1,Zhi-qiang HUANG1,Ying-lin WANG1,Ying YU1,Zhi-tao GU2,Qing LIU1,**()
1 School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
2 Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China
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Abstract  

Early screening and diagnosis of cancer is an effective means to reduce its incidence rate and mortality. Breast cancer is the most common malignant tumor among women, and has surpassed lung cancer to become the tumor with the highest incidence rate among women. With the innovation of electrochemical technology and the advantages of biosensors such as high sensitivity, rapid detection, simple operation and low cost, electrochemical biosensors are widely used in the detection of breast cancer markers. This review first summarizes the classification of common breast cancer biomarkers, and then summarizes and discusses the application of electrochemical biosensors in tumor markers of breast cancer at home and abroad, with electrochemical related immunosensors and aptamer sensors as the classification, and discusses their development prospects.

Key words： Breast cancer      Early detection      Tumor marker      Electrochemical sensor     
Received: 09 January 2023      Published: 03 August 2023
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Jia-ye JIANG
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Zhi-tao GU
Qing LIU
Cite this article:

Jia-ye JIANG, Ya-fang WU, Zhi-qiang HUANG, Ying-lin WANG, Ying YU, Zhi-tao GU, Qing LIU. Research Progress of Electrochemical Biosensor in the Detection of Tumor Markers in Breast Cancer. China Biotechnology, 2023, 43(7): 101-113.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2301008     OR     https://manu60.magtech.com.cn/biotech/Y2023/V43/I7/101

乳腺癌分型 相关靶标
HR(PR/ER)/HER2 其他相关检测靶标 代表性乳腺癌细胞系[33] 乳腺癌占比[34]
Luminal A HR+,HER2- Ki-67(低表达)[35] MCF-7 约50%
Luminal B HR+,HER2+ EGFR+[36]、Ki-67(高表达)[35] BT-474 约20%
基底样(三阴性乳腺癌) HR-,HER2- CK5/6+和/或HER1+[37] MDA-MB-231 约15%~20%
HER2过表达 HR-,HER2++ CA125、CA15-3[38] SK-BR-3 约10%~15%
Table 1 The classification of breast cancer subtypes and related markers
检测靶标 识别元件 传感技术 工作电极 动态范围 检测限 信号放大器 参考文献
HER2 抗体 DPV
CA		 MnO2
纳米片		 50 fg/mL~100 ng/mL,
100 fg/mL~100 ng/mL		 16.7 fg/mL,
33.3 fg/mL		 Au@Ag纳米棒 [73]
HER2-ECD 抗体 DPASV SPCE 10~150 ng/mL 2.1 ng/mL CdSe@ZnS量子点 [47]
HER2 抗体、肽段 AuE 1~200 pg/mL 90 fg/mL DNA链复制 [49]
HER2 适配体 PEC WS2 NW/
TM		 0.5~10 ng/mL 0.36 ng/mL 葡萄糖氧化酶酶促、金纳米
颗粒局部表面等离子体共振		 [74]
HER2-ECD
CA15-3		 抗体 SLV SPCE 0~50 ng/mL,
0~70 U/mL		 2.9 ng/mL
5.0 U/mL		 碱性磷酸酶酶促 [48]
CA15-3 抗体 ECL GCE 0.000 5~500 U/mL 0.000 2 U/mL Pt@BSA核/壳纳米球 [75]
MUC-1 抗体 CV PGE 0.05~940 U/mL 0.01 U/mL DA/MUC-1/fMWCNTs [76]
MUC-1 适配体 DPV
CA		 Au/GCE 1 fg/mL~10 pg/mL 0.72 fg/mL
0.82 fg/mL		 AuNPs@Cu7S4@Cu/
Mn-AzoPPOP)		 [77]
MUC-1 适配体 ECL GCE 1 pg/mL~10 ng/mL 0.23 pg/mL Zn-TP@Zn-Bp-MOFs [78]
MCF-7
(EpCAM)		 适配体、
抗体		 ECL GCE 1.1×102~1.1×107 U/μL 37 U/μL Ru(dcbpy ) 3 2 +@BPQDs [79]
SK-BR-3
(HER2)		 抗体 ECL GCE 20~2 000 cell/mL 20 cell/mL 鲁米诺/壳聚糖 [52]
MDA-MB-231 适配体 PEC CPE 300~5 000 cell/mL 180 cell/mL CNTs/SnSe [80]
MDA-MB-231 抗体 PEC ITO 1×102~1×106 cell/mL 30 cell/mL BiOBr/FeTPPCl/BiOI [81]
miRNA-21 核酸 DPV AU 1 fmol/L~1 nmol/L 0.29 fmol/L MOF@Pt@MOF纳米酶 [82]
Table 2 Selected examples of electrochemical sensors for detecting breast cancer tumor markers with performance
Fig.1 Electrochemical sensor for detecting HER2 (a) Electrochemical sensor for simultaneous detection of HER2-ECD and CA15-3[48] (b) Amplification of HER2 detection signal using RCA technology[49]
Fig.2 Electrochemical aptasensor for detecting mucin1[61]
Fig.3 Electrochemical aptasensor for detecting exosomes in breast cancer cells[70]
Fig.4 Nucleic acid electrochemical biosensor for detecting miRNA-122 [72]
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