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Effects of UPF1 on the Proliferation, Migration and Invasion of Breast Cancer Strains MDA-MB-231 and MCF-7 |
HU Kai,HU Jing,SUN Zi-jiu,LIU Shi-yan,LIAO De-yu,YU Huo-mei,ZHANG Yan() |
Key Laboratory of Clinical Laboratory Diagnosis, Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China |
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Abstract Objective: To investigate the effects of UPF1 on the proliferation, migration and invasion of human breast cancer cells MDA-MB-231 and its possible mechanism. Methods: The role of UPF1 in breast cancer and the expression level of UPF1 in pan-cancer and breast cancer was evaluated by bioinformatics analysis. Breast cancer cells MDA-MB-231 and MCF-7 were transfected with siRNA. The experiment was divided into two groups: control group (transfected with siRNA negative control) and treatment group (transfected with siUPF1). The mRNA and protein levels of UPF1, MMP9, EMT-related makers were measured by qRT-PCR and Western blot; the proliferation of MDA-MB-231 and MCF-7 cells was detected by CCK-8 assay; the lateral migration ability was studied by wound healing assay; the longitudinal migration and invasion abilities were evaluated by Transwell migration and invasion assays. Results: Bioinformatics analysis showed that UPF1 was highly expressed in breast cancer and associated with immune cell infiltration processes and positively correlated with tumor suppressor genes. UPF1 was also highly expressed in breast cancer cells. After knockdown of UPF1, the mRNA and protein expression levels of UPF1 in MDA-MB-231 and MCF-7 breast cancer cells were significantly decreased (P<0.05, P<0.05). Furthermore, the proliferation, migration and invasion abilities of MDA-MB-231 and MCF-7 cells were significantly enhanced. The mRNA and protein levels of MMP9 and Vimentin were increased, but E-cadherin decreased. Conclusion: UPF1 is highly expressed but plays a cancer-inhibiting role in breast cancer. UPF1 may inhibit the proliferation, migration and invasion of breast cancer MDA-MB-231 and MCF-7 cells by inhibiting the EMT signaling pathway.
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Received: 23 July 2021
Published: 03 March 2022
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Corresponding Authors:
Yan ZHANG
E-mail: yanzhang@cqmu.edu.cn
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