Identification of Microorganism Producing Humic Acids with Sugarcane Molasses Alcohol Waste Water and Optimum the Fermentation Conditions

China Biotechnology

2012, Vol. 32

Issue (10): 80-85 DOI:

Identification of Microorganism Producing Humic Acids with Sugarcane Molasses Alcohol Waste Water and Optimum the Fermentation Conditions

FENG Ling-bo¹, ZHOU Rui-fang¹, ZHAO Chen-long¹, CHEN Gui-guang¹, LI Yang-rui², LI Nan ^1,2,3

1. College of Life Science and Technology, Guangxi University, Nanning 530004, China;
2. Guangxi Academy of Agricultural Sciences, Nanning 530007, China;
3. Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning 530007, China

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Abstract The H812 strain was screened from sludge, it could make use of sugarcane molasses alcohol waste water to product humic acid, and it was preliminarily identified as Aspergillus sp by its culture characteristics, morphological features and ITS sequence analysis.The results of the monofactorial researchs and orthogonal experiments showed that 16°Bx was the optimal Bx of molasses alcohol waste water. The optimal fermentation conditions were: 8days, 34℃, 200r/min, pH7.0, inoculum size 12%, liquid volume 50ml/250ml,and the fermentation temperature had significant influence on the production of humic acid. At optimized fermentation conditions,HA content fermented with H812 strain was 38.12 g/L, and increased by 148. 34% than before optimization.

Key words： H812 strain Sugarcane molasses alcohol waste water Humic acid Identification Ferment conditions optimization

Received: 06 July 2012 Published: 25 October 2012

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FENG Ling-bo, ZHOU Rui-fang, ZHAO Chen-long, CHEN Gui-guang, LI Yang-rui, LI Nan. Identification of Microorganism Producing Humic Acids with Sugarcane Molasses Alcohol Waste Water and Optimum the Fermentation Conditions. China Biotechnology, 2012, 32(10): 80-85.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2012/V32/I10/80

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