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中国生物工程杂志

China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (12): 107-111    DOI: 10.13523/j.cb.20141215
    
Current Status and Application of Gene Trapping
WANG Ming-ke1,2,3, SUN Hui-qin2, SU Yong-ping2, ZOU Zhong-min4
1. Naval Medical Research Institute, Shanghai 200433, China;
2. State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China;
3. No. 441 Hospital of PLA, Fuding 355200, China;
4. Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China
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Abstract  

With the completion of genome sequencing on more and more organisms, post-genomic era has begun. Gene trapping, as a powerful tool in the functional-genomic era, has been widespreadly applicated in many fields of biomedicine research because of its great merits in cloning, finding novel genes and unraveling their biological functions. The principles, classifications, manipulation procedures, advantages and disadvantages of gene trap technique and its application in developmental biology, novel gene identification, stem cells differentiation, cancer and reproductive medicine research are discussed, which will provide helpful references for relevant researchers.



Key wordsGene trapping      Developmental biology      Novel gene identification      Stem cells differentiation      Cancer      Reproductive medicine     
Received: 08 October 2014      Published: 25 December 2014
ZTFLH:  Q789  
Cite this article:

WANG Ming-ke, SUN Hui-qin, SU Yong-ping, ZOU Zhong-min. Current Status and Application of Gene Trapping. China Biotechnology, 2014, 34(12): 107-111.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20141215     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I12/107


[1] Gentile A, D'Alessandro L, Medico E. Gene trapping: a multi-purpose tool for functional genomics. Biotechnol Genet Eng Rev, 2003, 20: 77-100.

[2] Skarnes W C, von Melchner H, Wurst W, et al. A public gene trap resource for mouse functional genomics. Nat Genet, 2004, 36 (6): 543-544.

[3] Stanford W L, Cohn J B, Cordes S P. Gene-trap mutagenesis: past, present and beyond. Nat Rev Genet, 2001, 2 (10): 756-768.

[4] 党素英, 王铸钢. 基因捕获技术. 国际遗传学杂志, 2006, 29 (1): 20-25. Dang S Y, Wang Z G. Gene-trapping technique. International Journal of Genetics, 2006, 29 (1): 20-25.

[5] 魏小慧, 郑肖兰, 郑服丛. 启动子捕获技术及其研究进展. 江西农业学报, 2009, 21 (4): 66-68. Wei X H, Zheng X L, Zheng F C. Promoter capturing technique and its research progress. Acta Agriculturae Jiangxi, 2009, 21 (4): 66-68.

[6] 万海英, 汤华. 基因敲除技术现状及应用. 医学分子生物学杂志, 2007, 4 (1): 86-90. Wan H Y, Tang H. Status quo and application of gene knockout. Journal of Medical Molecular Biology, 2007, 4 (1): 86-90.

[7] 龚强,胡维新. 基因诱捕技术及基因诱捕数据库. 生命的化学, 2007, 27 (1): 84-86. Gong Q, Hu W X. Gene trap and gene trap database. Chemistry of Life, 2007, 27 (1): 84-86.

[8] Skarnes W C, Auerbach B A, Joyner A L. A gene trap approach in mouse embryonic stem cells: the lacZ reported is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes Dev, 1992, 6 (6): 903-918.

[9] Tsakiridis A, Tzouanacou E, Rahman A, et al. Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells. Nucleic Acids Res, 2009, 37 (19): 1-14.

[10] Shirasawa S, Yoshimi M, Kamochi H, et al. Gene trap screening for cell surface and extracellular matrix molecules produced by chondrocytes. J Biochem, 2005, 137 (1): 79-85.

[11] 孙强, 韩骅. 分泌蛋白特异性基因陷阱的设计与验证. 生物化学与生物物理进展, 2004, 31 (4): 328-333. Sun Q, Han H. Design and efficacy of a gene trap system for secretory proteins. Progress in Biochemistry and Biophysics, 2004, 31 (4): 328-333.

[12] Chen Y T, Liu P, Bradley A. Inducible gene trapping with drug-selectable markers and Cre/loxp to identify developmentally regulated genes. Mol Cell Biol, 2004, 24 (22): 9930-9941.

[13] Chaiyachati B H, Kaundal R K, Zhao J, et al. LoxP-FRT Trap(LOFT): a simple and flexible system for conventional and reversible gene targeting. BMC Biol, 2013, 10: 96.

[14] Ni T T, Lu J, Zhu M, et al. Conditional control of gene function by an invertible gene trap in zebrafish. Proc Natl Acad Sci U S A, 2012, 109 (38): 15389-15394.

[15] Song G, Li Q, Long Y, et al. Effective expression-independent gene trapping and mutagenesis mediated by Sleeping Beauty transposon. J Genet Genomics, 2012, 39 (9): 503-520.

[16] Ullrich M, Schuh K. Gene trap: knockout on the fast lane. Methods Mol Biol, 2009, 561: 145-159.

[17] Schnutgen F, Ehrmann F, Ruiz-Noppinger P, et al. High throughput gene trapping and postinsertional modifications of gene trap alleles. Methods, 2011, 53 (4): 347-355.

[18] Springer P S. Gene traps: tools for plant development and genomics. Plant Cell, 2000, 12 (7): 1007-1020.

[19] 黄小乐. 面向后基因组研究的基因陷阱技术. 中山大学研究生学刊:自然科学与医学版, 2003, 24 (3): 6-15. Huang X L. Gene trap strategies for postgenome era. Journal of the Graduates Sun Yat-Sen University(Natural Sciences、Medicine), 2003, 24 (3): 6-15.

[20] Cecconi F, Meyer B I. Gene trap: a way to identify novel genes and unravel their biological function. FEBS Lett, 2000, 480 (1): 63-71.

[21] Akiyama N, Matsuo Y, Sai H, et al. Identification of a series of transforming growth factor beta-responsive genes by retrovirus-mediated gene trap screening. Mol Cell Biol, 2000, 20 (9): 3266-3273.

[22] Lako M, Hole N. Searching the unknown with gene trapping. Expert Rev Mol Med, 2000, 2 (5): 1-11.

[23] Evans M J, Carlton M B, Russ A P. Gene trapping and functional genomics. Trends Genet, 1997, 13 (9): 370-374.

[24] 汤华,唐任宽. 利用捕获载体解析小鼠基因在生物发育过程中的功能. 生命的化学, 2005, 25 (1): 49-51. Tang H, Tang R K. Unraveling gene function in mouse development by trapping vector. Chemistry of Life, 2005, 25 (1): 49-51.

[25] Osokine I, Hsu R, Loeb G B, et al. Unintentional miRNA ablation is a risk factor in gene knockout studies: a short report. PLoS Genet, 2008, 4 (2): e34.

[26] Gossler A, Joyner A L, Rossant J, et al. Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science, 1989, 244 (4903): 463-465.

[27] Kohoutek J, Li Q T, Blazek D, et al. Cyclin T2 Is Essential for mouse embryogenesis. Mol Cell Biol, 2009, 29 (12): 3280-3285.

[28] Brukhin V B, Jaciubek M, Bolanos Carpio A, et al. Female gametophytic mutants of Arabidopsis thaliana identified in a gene trap insertional mutagenesis screen. Int J Dev Biol, 2011, 55 (1): 73-84.

[29] Springer P S, Holding D R, Groover A, et al. The essential Mcm7 protein PROLIFERA is localized to the nucleus of dividing cells during the G(1) phase and is required maternally for early Arabidopsis development. Development, 2000, 127 (9): 1815-1822.

[30] Yu S M, Ko S S, Hong C Y, et al. Global functional analyses of rice promoters by genomics approaches. Plant Mol Biol, 2007, 65 (4): 417-425.

[31] Tang H, Araki K, Yamamura K. Cloning and expression analysis of a murine novel gene, Ayu17-449. Yi Chuan Xue Bao, 2006, 33 (5): 413-419.

[32] Forbes J M, Ke B X, Nguyen T V, et al. Deficiency in mitochondrial complex I activity due to ndufs6 gene trap insertion induces renal disease. Antioxid Redox Signal, 2013, 19 (4): 331-343.

[33] Hirashima M, Bernstein A, Stanford W L, et al. Gene-trap expression screening to identify endothelial-specific genes. Blood, 2004, 104 (3): 711-718.

[34] 王明科, 邹仲敏, 罗成基, 等. 多用途基因捕获C3H/10T1/2细胞阳性克隆库的构建及鉴定. 第四军医大学学报, 2008, 29 (6): 508-512. Wang M K, Zou Z M, Luo C J, et al. Establishment and identification of gene trap clones from C3H/10T1/2 cells transfected with rosafary vector. Journal of Fourth Military Medical University, 2008, 29 (6): 508-512.

[35] Wang M, Sun H, Jiang F, et al. Cloning and characterization of a novel gene with alternative splicing in murine mesenchymal stem cell line C3H/10T1/2 by gene trap screening. BMB Rep, 2010, 43 (12): 789-794.

[36] 王明科, 姜帆, 孙慧勤, 等. 基因捕获筛选 TGF-β1 诱导间充质干细胞 C3H/10T1/2 平滑肌分化的差异表达基因. 第三军医大学学报, 2014, 36 (2): 92-97. Wang M K, Jiang F, Sun H Q, et al. Identification of differentially expressed genes upon TGF-β1 induced smooth muscle differention of mesenchymal stem cell lines C3H/10T1/2 cells by gene trap screening. Journal of Third Military Medical University, 2014, 36 (2): 92-97.

[37] 王明科, 孙慧勤, 程晋, 等. 新基因 mgt-16 反转录病毒载体的构建及其在小鼠间充质干细胞中的表达. 第二军医大学学报, 2014, 35 (4): 447-451. Wang M K, Sun H Q, Cheng J, et al. Construction of retrovirol vector containing novel gene mgt-16 and its expression in mouse mesenchymal stem cells. Academic Journal of Second Military Medical University, 2014, 35 (4): 447-451.

[38] Ishiguro T, Avila H, Lin S Y, et al. Gene trapping identifies chloride channel 4 as a novel inducer of colon cancer cell migration, invasion and metastases. Brit J Cancer, 2010, 102 (4): 774-782.

[39] Yamamura K, Araki K. Gene trap mutagenesis in mice: new perspectives and tools in cancer research. Cancer Sci, 2008, 99 (1): 1-6.

[40] Tarnasky H, Cheng M, Ou Y, et al. Gene trap mutation of murine outer dense fiber protein-2 gene can result in sperm tail abnormalities in mice with high percentage chimaerism. BMC Dev Biol, 2010, 10: 67.

[41] Kanatsu-Shinohara M, Ikawa M, Takehashi M, et al. Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells. Proc Natl Acad Sci U S A, 2006, 103 (21): 8018-8023.

[42] Gow M, Mirembe D, Longwe Z, et al. A gene trap mutagenesis screen for genes underlying cellular response to the mood stabilizer lithium. J Cell Mol Med, 2013, 17 (5): 657-663.

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