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| Pilot Study on Bone Marrow Mesenchymal Stem Cells Cultured with Biological Materials P (3HB-co-4HB) as Cell Patch |
| NIU Hong-xing1, MU Jun-sheng1, ZHANG Jian-qun1, HU Ping2, BO Ping1, WANG Yang 2 |
1. Department of Cardiac the Affiliated AnZhen Hospital of Capital University of Medical Science, Beijing 100029, China;
2. Department of Chemical Industry Qinghua University, Beijing 100084, China |
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Abstract Objective: To study poly 3-hydroxybutyrate-co-4-hydroxybutyrate[P(3HB-co-4HB)] new polymer materials co-cultured with bone marrow mesenchymal stem cells, observe the impact of materials of cell proliferation, find a polymer biomaterials fit for the stem cell survival, as one of the treatment of variety of diseases patch. Method:Take clean male healthy BSL-C57 mice as an experiment animals, use the 3rd generations through isolated and cultured for murinev and the identification with flow cytometry. Use 3rd generation bone marrow mesenchymal stem cell and P (3HB-co-4HB) made of biological material film a total of culture, After 24h fixed conducted scanning electron microscopy, and using DAPI fluorescent dye staining processing observed by fluorescence microscopy and cell count, and describe the counting growth curve. Results: The result of flow cytometry identification is CD34, CD45 negative, CD90 weakly positive, CD73 positive. The scanning electron microscope,at P (3HB-co-4HB) surface a large number of normal cell state. Draw the growth curve with cell counting.Mean that the surface of cells is a growing tendency. Conclusion: P (3HB-co-4HB) materials and BMSCs cultured made cell patch surface cell survival, proliferation, due to P (3HB-co-4HB), the material itself has a good biological histocompatibility and biodegradable nature, became one of the good materials for cell therapy.
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Received: 26 June 2012
Published: 25 October 2012
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[1] Carolyn J. Teng, Luo Jun, Ray C J, et al. Massive mechanical loss of microspheres with direct intra myocardial injection in the beating heart: Implications for cellular cardiomyoplasty. J Thorac Cardiov Sur, 2006,132(3 ):628-632.
[2] Adil H A, Juan Francisco Asenjo, Yin Ge, et al.Microencapsulation to reduce mechanical loss of microspheres: implications in myocardial cell therapy. Euro J Cardio-Thorac Surg, 2011,39(2):241-247.
[3] Saito T, Kuang J Q, Lin C C, et al. Transcoronary implantation of bone marrow stromal cells ameliorates cardiac function after myocardial infarction Journal of Thoracic and Cardiovascular Surgery, 2003,126( 1): 114-122.
[4] Takayuki Saito, Jin Q K, Charles C H, et al. Transcoronary implantation of bone marrow stromal cells ameliorates cardiac function after myocardial infarction. J Thorac Cardiov Sur, 2003,126( 1): 114-122.
[5] Kopen G C, Prockop D J, Phinney D G. et al. Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains. Proc Natl Acad Sci USA, 1999,96 (19):10711 -10716.
[6] Jian-Ye Zhou, Zhe Zheng, Hao Zhang, et al. A novel vascularized patch enhances cell survival and modifies ventricular remodeling in a rat myocardial infarction model. The Journal of Thoracic and Cardiovascular Surgery, 2010, 140( 6):1388-1396.
[7] Mani T V, Richard L Goodwin, John W Fuseler, et al. A 3-D cardiac muscle construct for exploring adult marrow stem cell based myocardial regeneration. Biomaterials, 2010, 31 (12):3185-3200.
[8] Torun K, Gand K F. Bone generation on P(3HB-CO-4HB) matrices: an in vitro study. Biomaterials, 2003,24: 4999-5007.
[9] Michal Shachar, Orna Tsur-Gang, Tal Dvir, et al. The effect of immobilized RGD peptide in alginate scaffolds on cardiac tissue engineering. Acta Biomaterialia, 2011,7(1):152-162.
[10] Bong-Wook Park, Eun-JuKang, June-HoByun, et al. In vitro and in vivo osteogenesis of human mesenchymal stem cells derived from skin,bone marrow and dental follicle tissues. Differentiation, 2012,(83):249-259.
[11] Michal S, Orna Tsur-Gang, Tal Dvir, et al.The effect of immobilized RGD peptide in alginate scaffolds on cardiac tissue engineering. Acta Biomaterialia, 2011, 7 (1):152-162.
[12] Saeed H, Taipaleenmäki H, Aldahmash A M, et al. Mouse embryonic fibroblasts (MEF) exhibit a similar but not identical phenotype to bone marrow stromal stem cells (BMSCS). Stem Cell Rev, 2012,8(2):318-328.
[13] Zhang S, Ge J, Sun A, et al. Comparison of various kinds of bonemarrow stem cells for the repair of infarcted myocardium: single clonallypurified non-hematopoietic mesenchymal stem cells serve as a superior source. J Cell Biochem, 2006,99(4):1132-1147.
[14] Jain M, Pfister O, Hajjar R J, et al. Mesenchymal stem cells in the infarcted heart. Coron Artery Dis, 2005,16(2):93-97.
[15] Pittenger M F, Mackay A M, Beck S C,et al.Multilineage potential of adult human mesenchymal stem cells. Science, 1999, 284 (5411): 143.
[16] Martens T P, See F, Schus T. et al. Mesenchymal lineage precursor cells induce vascular network formation in ischemic myocardium. Nat Clin Pract Cardiovasc Med, 2006,3(1):18-22.
[17] Kindi A H, Asenjo J F, Ge Y, et al. Micro encapsulation to reduce mechanical loss of microspheres: implications in myocardial cell therapy. Eur J Cardio thorac Surg, 2011, 39(2): 241-247.
[18] Tobella L M,Btmster M,Pooley A.Biosynthesis of poly-beta-hydroxyalkanoates by Sphingopyxis chilensis S37 and Wautersia sp.PZK cultured in cellulose pulp mill effluents containing 2,4,6-trichloro phenol.J Ind Microbiol Biotechnol,2005,32(9):397.
[19] Thakor N,Trivedi U,Pate1 K C,et al.Biosynthesis of medium chain length poly(3-hydroxyalkanoates) (mcl-PHAs) by Comamonas testosteroni during cultivation on vegetable oils.Bioresour Technol,2005,96(17):1843. |
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