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| Clonning the Promoter of AtHsp17.6-C2 and its Heat Inducible Kinetics |
| ZENG Wei-jun1,2,WANG Shui-ping1,LI Xiao-fang1,XU Ping3,WANG Rui-gang4** |
1.School of Life Science, East China Normal University, Shanghai 200062, China
2.College of Life Science and Chemistry,Xinjiang Normal University,Urumqi 830054,China
3.Shanghai Information Center for Life Science,CAS, Shanghai 200031,China
4.College of Life Sciences, Inner Mongolia Agricultural University, Huhhot 010018, China |
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Abstract A gene expression system were constructed, driven by a heat-shock gene promoter (AtHsp17.6-C2), to control the expression of reporter gene (GUS) in transgenic plants. The expression of the AtHsp17.6-C2 chimeric gene in the stable transformants of ⊿F122-13 was hardly detected in culture at 22℃. However,the expression increased dramatically at the transcriptional level when the incubation temperature was shifted to 34~37℃. The optimal temperature for heat (shock) induction was 37℃. After a 2h incubation at 37℃ and 2h recovery phase at 22℃, GUS activity was about 80 fold greater than that before heat shock. Multiple heat-shock treatments showed that this system was heat reinducible. This new system can be applied in numerous and various applications.
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Received: 21 January 2010
Published: 12 June 2010
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