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Heterologous Expression of CiRS Gene Eenhances the Antioxidant Capacity of Arabidopsis by Increasing the Content of Resveratrol |
Fei-yun YANG1,2,Yan-yan WU2,Shuang CUI1,Xiu-juan ZHANG3,Rui-gang WANG1,Guo-jing LI1() |
1 College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China 2 College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China 3 Inner Mongolia Autonomous Region Biotechnology Research Institute, Hohhot 010070, China |
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Abstract Resveratrol is a member of stilbenoids with disease resistant activity for plants and exhibits a wide range of important biological and pharmacological properties for human which has received extensive attention in the fields of agriculture, medicine, foods, cosmetics and so on. Resveratrol synthase (RS) is an exclusive necessary enzyme in the pathway of resveratrol biosynthesis which determines the synthesis of resveratrol in plants. CiRS, a RS gene isolated from Caragana intermedia, was transferred into Arabidopsis. Total flavonoids experimental results showed that the total flavonoids content of wild type was significantly higher than that of the transgenic lines. HPLC method was used to analyze the resveratrol content in transgenic plants which maximum content was 335μg/g FW. Accumulation of malondialdehyde (MDA) after UV treatment in transgenic plants was significantly less than the wild type. DPPH free radical scavenging ability of transgenic plants extraction was higher than the wild type. Taken together, these results indicated that the antioxidant activity of transgenic plants was enhanced with the expression of CiRS gene which synthesise resveratrol with flavonoid substrates.
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Received: 24 August 2017
Published: 16 December 2017
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