Application of Tn5 Transposon Mutagenesis Technology in Molecular and Genetic Researches of Gramnegative Bacteria

China Biotechnology

2009, Vol. 29

Issue (12): 114-118 DOI:

Application of Tn5 Transposon Mutagenesis Technology in Molecular and Genetic Researches of Gramnegative Bacteria

NIAN Hong-juan,CHEN Li-mei,LI Kun-zhi

Biotechnology Research Center, Kunming University of Science and Technology,Kunming 650224,China

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Abstract

With development of wide-host-range vector systems, Tn5 transposon and its derivative vectors have been widely applied to genetic research of gram-negative bacteria.The applications of Tn5 transposon mutagenesis technology to genetic researches of bacteria were briefly discussed, including researches on biological control mechanisms of biocontrol bacteria, identification of bacterial essential genes, discovering virulence genes of bacterial pathogens, characterization of metabolism regulatory genes and genetic improvements of bacteria.

Key words： Tn5 transposon mutagenesis Biocontrol mechanism Bacterial essential geneVirulence gene Metabolism regulatory gene Bacteria improvements

Received: 06 May 2009 Published: 21 December 2009

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	NIAN Hong-Juan
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NIAN Hong-Juan, CHEN Li-Mei, LI Hun-Zhi. Application of Tn5 Transposon Mutagenesis Technology in Molecular and Genetic Researches of Gramnegative Bacteria. China Biotechnology, 2009, 29(12): 114-118.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I12/114


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