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| Cloning and Transient Expression Analysis of PtSEP2 Promoter from Populus tomentosa |
| CUI Dong-qing, YE Mei-xia, LIU Jun-mei, LI Hao, ZHANG Zhi-yi, AN Xin-min |
| National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education; The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Beijing Forestry University, Beijing 100083, China |
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Abstract A 5' flanking sequence of PtSEP2 which is a SEPALLATA2 -like gene involved in floral organ development was amplified by PCR from the genomic DNA of Populus tomentosa. The length of fragment is approximately 2.3kb. The results derived from PlantCARE analysis showed that the sequence contains conserved cis-acting elements of promoter, coupled with a variety of light-responsive elements. Therefore, it was speculated preliminarily to be the promoter of PtSEP2 gene. To investigate function of this promoter, it was fused to GUS reporter gene, generating a plant expression vector pPtSEP2 promoter∷GUS, named PtSEP2p∷GUS . With roots, stems, leaves and flower buds of Nicotiana tobaccum for the receptor, the results of transient expression that Agrobacterium-mediated that showed the PtSEP2 promoter was competent to drive specially GUS reporter gene expression in anther, yet its’ activity was weaker than that of cauliflower mosaic virus (CaMV) 35S promoter, which constitutively expressed in planta. Consequently, provides a possible genetic modification tool for flowering regulation in poplar and other plants.
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Received: 24 November 2010
Published: 27 May 2011
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