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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (1): 29-37    DOI: 10.13523/j.cb.20160105
    
Cloning and Expression Analysis of Rice miRNA3026 Promoter and Thioredoxin OsTxnDC9
DONG Juan, LI Fo-sheng, LUO Feng-Xue, XIA Fang, ZHU Shu-hua, TANG Lin
College of Life Science, Sichuan University, Chengdu 610064, China
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Abstract  

The 1 477bp promoter of miRNA3026 was cloned, furthermore, four mutant fragments with different length of deletion were constructed and transient expression assay showed that the activity of miRNA3026 promoter is weak. OsTxnDC9 was cloned, the host gene of miRNA3026, which has an open reading frame of 482bp. Bioinformatic analysis indicated that OsTxnDC9 encodes a protein which is highest homology with Brachypodium distachyon. The expression of OsTxnDC9 was highest in one nuclear stage of pollen, which was examined by real-time polymerase chain reaction essay. Subcellular localization suggested that the protein was located in cytoplasm. This research laied the foundation for further exploring the relationship of miRNA3026 promoter and its host gene.



Key wordsPromoter      Real-time polymerase chain reaction      Thioredoxin      Transient expression      Host gene     
Received: 19 October 2015      Published: 11 January 2016
ZTFLH:  Q945  
Cite this article:

DONG Juan, LI Fo-sheng, LUO Feng-Xue, XIA Fang, ZHU Shu-hua, TANG Lin. Cloning and Expression Analysis of Rice miRNA3026 Promoter and Thioredoxin OsTxnDC9. China Biotechnology, 2016, 36(1): 29-37.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160105     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I1/29

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