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Cloning and Expression Analysis of Rice miRNA3026 Promoter and Thioredoxin OsTxnDC9 |
DONG Juan, LI Fo-sheng, LUO Feng-Xue, XIA Fang, ZHU Shu-hua, TANG Lin |
College of Life Science, Sichuan University, Chengdu 610064, China |
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Abstract The 1 477bp promoter of miRNA3026 was cloned, furthermore, four mutant fragments with different length of deletion were constructed and transient expression assay showed that the activity of miRNA3026 promoter is weak. OsTxnDC9 was cloned, the host gene of miRNA3026, which has an open reading frame of 482bp. Bioinformatic analysis indicated that OsTxnDC9 encodes a protein which is highest homology with Brachypodium distachyon. The expression of OsTxnDC9 was highest in one nuclear stage of pollen, which was examined by real-time polymerase chain reaction essay. Subcellular localization suggested that the protein was located in cytoplasm. This research laied the foundation for further exploring the relationship of miRNA3026 promoter and its host gene.
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Received: 19 October 2015
Published: 11 January 2016
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[1] Wu C, You C, Li C, et al . RID 1,encoding a Cys2/His2-type zinc finger transcription factor, acts as a master switch from vegetative to floral development in rice. Proc Natl Acad Sci USA,2008,1059(2): 12915-12920.
[2] Li X, Yang Y, Yao J, et al . FLEX IBLE CULM 1 encoding a cinnamyl-alcohol dehydrogenase controlsculm mechanical strength in rice.Plant Mol Biol,2009,69(6): 685-697.
[3] Li X, Qian Q, Fu Z, et al . Control of tillering in rice. Nature, 2003, 422(9): 618-621.
[4] Ren Z, Gao J, Li L, et al . A rice quantitative trait locus for salt to lerance encodes a sodium transporter. Nat Genet, 2005, 37(5): 1141-1146.
[5] Sun X, Cao Y, Yang Z, et al. Xa26,a gene conferring resistance to X anthom on as Oryzae pv. Plant,2004, 37(4): 517-527.
[6] Huang X, Qian Q, Liu Z, et al . Natural variation at the DEP1 locus enhances grain yield in rice. Nat Genet, 2009, 41(7): 494-497.
[7] Heide H, Kalisz H M, Follmann H .The oxygen evolving enhancer protein 1 (OEE) of photosystem Ⅱ in green algae exhibits thioredoxin activity.Plant Physiol,2006,161(2): 139-149.
[8] Laurent T C,Moore E C,Reichard P.Enzymatic synthesis of deoxyribonucleotides .Journal of Biological Chemistry,1964,239(10):3436-3444.
[9] Gelhaye E, Rouhier N,Navrot N,et al.The plant thioredoxin system.Cell Mol,2005,62(1):24-35.
[10] Nishiyama A,Masutani H,Nakamura H,et al.Redox regulation by thioredoxin and thioredoxin binding proteins. Cell Mol,2004, 52 (1): 29-33.
[11] Radfar M H, Wong W, Morris Q. Computational prediction of intronic microRNA targets using host gene expression reveals novel regulatory mechanisms. PLoS One, 2011, 6(6): 193-202.
[12] Laufs P, Peaucelle A, Morin H, et al. MicroRNA regulation of the CUC genes is required for boundary size control in Arabidopsis meristems. Development, 2004, 131(17): 4311-4322.
[13] Jones-Rhoades M W, Bartel D P. Computational identification of plant microRNAs and their targets, including a stress-induced miRNA. Molecular Cell, 2004, 14(6): 787-799.
[14] Sunkar R, Zhu J. Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis. The Plant Cell Online, 2004, 16(8): 2001-2019.
[15] Yoo S D, Cho Y H.Arabidopsis mesophyll protoplasts:a versatile cell system for transient gene expression analysis.Protocol,2007,7(2):1565-1572.
[16] Langhner B J,Sehnke P C,Ferl R J.A novel nuclear member of the thioredoxin superfamily .Plant Physiology,1998,118(9):987-996.
[17] Wong J H,Cai N,Balmer Y,et al.Thioredoxin targets of developing wheat seeds identified by complementary proteomic approaches.Phytochemistry,2004,65(6):1629-1640.
[18] 李巧云,牛洪斌,任江萍,等.转外源Trxs基因大麦耐盐性有关生理生化特性分析.作物,2009,118(5):7-10. Li Q Y,Niu H B,Ren J P,et al.Effects of exogenous Trxs on physiological and biochemical characteristics of salt tolerance in transgenic barley seedlings.Crops,2009,118(5):7-10.
[19] 王玉成, 杨传平, 刘桂丰, 等.紫杆柽柳cDNA文库构建与硫氧还蛋白基因的克隆.分子植物育种,2004, 2(5): 667-673. Wang Y C,Yang C P,Liu G F,et al.Construction of Tamarix androssowii cDNA library and cloning of thioredoxin gene.Molecular Plant Breeding,2004,2(5):667-673.
[20] Tanaka T, Hosoi F, Yamaguchi-Iwai Y, et al. Thioredoxin-2 (TRX-2) is an essential gene regulating mitochondria-dependent apoptosis. Plant Physiology, 2002,21(7): 1695-1703.
[21] 曹丽,黄大无,范慧,等.人硫氧还蛋白在大肠杆茵中的高效表达、 纯化反对内毒素血症小鼠的保护作用.中国生物化学与分子生物学报,2002,18(6):733- 736. Cao L,Huang D W,Fan H,et al.The Expression and purification of human thioredoxin from E.coli and Its protective effects in a mutine model of endotoxemia.Chinese Journal of Biochemistry and Molecular Biology,2002,18(6):733-736.
[22] Shibuki H, Katai N, Kuroiwa S, et al.Protective effect of adult T-cell leukemi-a-derived factor on retinal ischemia-reperfusion injury in the rat. Molecular Cell, 1998,39(8):1470-1477.
[23] Hirota K, Murata M, Sachi Y, et al. Distinct roles of thioredoxin in the cytoplasm and in the nucleus: a two-step mechanism of re-dox regulation of transcription factor NF-κB .Protocol,1999,2(5): 27891-27897. |
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