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| The Cloning and Transient Expression Analysis of Promoter of OsHAK26 from Oryza sativa |
| LUO Feng-xue, LI Fo-sheng, YAO Min, XU Ying |
| Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China |
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Abstract A 2 064 bp upstream sequence of translation initiation site of OsHAK26, a member of KT/HAK/KUP potassium transporters gene family in rice was analyzed and that included a number of elements in response to the development, hormones, and abiotic stresses and common promoter elements of KT/HAK/KUP family, in addition to the basic promoter elements such as TATA-Box, CAAT-Box and so on. Subsequently, the fragment together with other four different 5'-end deletion fragments (－1 473bp、－963bp、－441bp、－193bp) of the OsHAK26 gene 5'-flanking sequences were inserted into the plant transient expression vector pBI221 to replace CaMV 35S promoter respectively and the transient expression analysis was conducted in Arabidopsis mesophyll protoplast system. The result showed that the five fragments had promotor activity decreasing with the length decrease. but deletion of －963bp~－441bp had led to a significant rebound in activity, so it inferred that the section contained suppression components.－441bp~+95bp region activity suddenly surged,so the －441bp~－193bp was core region of promoter of OsHAK26.
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Received: 19 August 2016
Published: 25 February 2017
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