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| Effect of RMP Gene on Genome Stability and Apoptosis of Hepatoma Carcinoma Cells |
| WANG Xiao-tin, LI Min, LIAN Xiao-nin, YANG Hui-cui, CAO Kai, GUO Yun-lan, ZHONG Lei, WEI Weng-xiang |
| Department of Cellular and Molecular Biology,Medical Suzhou University,Suzhou 215123,China |
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Abstract Objective: To explore the effect of RPB5-Mediating Protein on cell genome stability and apoptosis of human hepatocellular carcinoma cell. Methods:RT-PCR was used to detect the expression of RMP mRNA on normal cell lines and several tumour cell lines. The SMMC-7721 cells and human hepatic cell HL-7702 cells were exposed to60Co γ-ray of different dose; then semiquantitative RT-PCR assay was used to detect the expression of RMP; Flow cytometric technique were used to detect apoptosis and cell cycle. To study the effect of RMP on cell genome stability and apoptosis of human hepatocellular carcinoma cell by RNA interfere . Results:RMP could express in different normal cell lines and tumour cell lines. The expression of RMP gene increased with the increase of exposure dosage. Flow cytometry results also indicated that cell apoptosis increased with increasing dosage, with the number of cells in G1 stage increased and that in S stage decreased. After RMP gene was interfered, the morphocytology of the cells changed obviously, meanwhile, the expression of p21 decreased. Conclusions:RMP may have a potential role on maintaining cell genome stability. RMP may associate with p53、p21, which have an role on regulating apoptosis.
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Received: 23 July 2010
Published: 26 April 2011
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[1] Cheong J H, Yi M, Lin Y,et al. Human RPB5, a subunit shared by eukaryotic nuclear RNA polymerases, binds human hepatitisB virus X protein and may play a role in X transactivation.EMBO J,1995,14(1):143-150.
[2] Dorjsuren D, Lin Y, Wei W,et al. RMP, a novel RNA polymerase II subunit 5-interacting protein, counteracts transactivation by hepatitis B virus X protein.Mol Cell Biol, 1998,18(12):7546-7555.
[3] Delgermaal, Hayashi N, Dorjsuren D, et al. Subcellular localization of RPB5-mediating protein and its putative functional partner.Mol Cell Biol,2004, 24(19):8556-8566.
[4] Wei W, Gu J X, Zhu C Q,et al.Interaction with general transcription factorIIF(TFIIF)is required for the suppression of activated transcription by RPB5-mediating protein(RMP) .Cell Res,2003,13(2):111-120.
[5] Tang H, Oishi N, Kaneko S,et al. Molecular function and biological roles of hepatitis B virus x protein.Cancer Sci, 2006 ,97(10):977-983.
[6] Keasler V V, Hodgson A J, Madden C R, et al. Enhancement of hepatitis B virus replication by the regulatory X protein in vitro and in vivo. J Virol,2007,81(6):2656-2662.
[7] Lee A T, Ren J,Wong E T, et al .The hepatitis B virus X protein sensitizes HepG2 cells to UV light-induced DNA damage.J Biol Chem,2005,280(39):33525-33535.
[8] Morrison A J,Shen X. DNA repair in the context of chromatin. Cell Cycle,2005, 4(4):568-571.
[9] Christine T P, Ekaterini A K, Michael O H,et al. URI-1 is required for DNA stability in C. elegans.Development ,2006,133(4):621-629.
[10] Quevedo C, Kaplan D R, Derry W B. AKT-1 regulates DNA-damage-induced germline apoptosis in C. elegans. Curr Biol, 2007,17(3):286-292.
[11] Salinas L S, Maldonado E, Navarro R E. Stress-induced germ cell apoptosis by a p53 independent pathway in Caenorhabditis elegans. Cell Death Differ, 2006,13(12):2129-2139.
[12] Arenz C, Schepers U. RNA interference: from an ancient mechanism to a state of the art therapeutic application. Naturwissenschaften, 2003, 90(8):345-359.
[13] Ryther R C, Flynt A S, Phillips J A, et al. siRNA therapeutics: big potential from small RNAs. Gene Ther,2005, 12(1):5-11.
[14] Joshua-Tor L. siRNAs at RISC. Structure, 2004, 12(7):1120-1122.
[15] Reynolds A, Leake D, Boese Q, et al. Rational siRNA design for RNA interference. Nat Biotechnol, 2004,22(3):326-330.
[16] Gartel A L,Radhakrishnan S K.Lost in transeription:p2l repressionmechanisms,and consequences.Cancer Res,2005,65(10):3980-3985.
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