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| Establishment of the Transgenic Mice Expressing the HBV Receptor Human ASGPR |
| GUO Hong-min1,2,WU Xiao-Jie2,LI Xiang3,ZHOU Yan-rong2,LIN Yan-li2,XIONG Fu-yin2,XUE Shi-wei1,CHEN Hong-xing2,CHEN Shu-lin1 |
1.College of Animal Medicine,Northwest A&F University,Yangling 712100,China
2.Institute of Biotechnology , Academy of Military Medical Sciences, Beijing 100071,China
3.College of Life Science,Shannxi Normal University,Xi’an 710062,China |
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Abstract Objective:To generate the transgenic mice that express human Asialoglycoprotein receptor(ASGPR),which is considered as one of the hepatitis B virus (HBV) receptors. Methods: Both cDNA of the Human ASGPR H1&H2 subunits were cloned and the expression vector PCAGGS-ASGPRH1&H2 were constructed, Both of the 3.9kb DNA fragments were introduced into the fertilized eggs of mice by microinjection. The genotypes of the transgenic mice were identified by PCR and Southern-blot methods, and the expression of the exogenous ASGPR gene was confirmed by the RT-PCR and Western blot. Result and Conclusion: One transgenic mouse line that express the human ASGPR was obtained. It can be used as an animal model for the research of HBV infection.
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Received: 13 January 2010
Published: 25 May 2010
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Cite this article:
GUO Gong-Min, TUN Xiao-Ji, LI Xiang, ZHOU Yan-Rong, LIN Yan-Li, XIONG Fu-Yin, XUE Shi-Wei, CHEN Gong-Xing, CHEN Shu-Lin. Establishment of the Transgenic Mice Expressing the HBV Receptor Human ASGPR. China Biotechnology, 2010, 30(05): 87-91.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I05/87
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