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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (4): 97-103    DOI: 10.13523/j.cb.20160414
    
Construction of SND1 Transgenic Mice
ZUO Zhi-yu1, XIN Ling-biao1, YANG Jie1,2, WANG Xin-ting1,2
1. Basic Medical College of Tianjin Medical University, Department of Immunization, Tianjin 300070, China;
2. Basic Medical College of Tianjin Medical University, Department of Biochemistry and Molecular Biology, Tianjin 300070, China
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Abstract  

Objective: The aim is to establish an animal model of SND1 over-expression transgenic mice. Methods: The mice SND1 gene transcripts were used for form pInsulator-CAG-3×FLAG-SND1 recombinant vector. Then injected the recombinant vector into fertilized egg by microinjection technology to acquire the transgenic mice. These offspring were identified by PCR. Results: SND1 transgenic mice was constructed successfully and expressed effectively on account of target gene can be transferred by the transgenic mice which was proved by PCR and RT-PCR.These mice could provide an important model for studying the biological function of SND1 gene in vivo.



Key wordsTransgenic mice      Vector construction      SND1     
Received: 30 October 2015      Published: 04 January 2016
ZTFLH:  Q789  
Cite this article:

ZUO Zhi-yu, XIN Ling-biao, YANG Jie, WANG Xin-ting. Construction of SND1 Transgenic Mice. China Biotechnology, 2016, 36(4): 97-103.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160414     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I4/97

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