China Biotechnology

Biopanning of Anti c-Myc-tag Nanobodies and Its Application for Bioimaging

Jin-jing LI,Fei XU,Yan-wei JI,Mei SHU,Zhui TU,Jin-heng FU

China Biotechnology, 2018, 38(2): 61-67. DOI: 10.13523/j.cb.20180209

Fig.6 Indirect ELISA for the protein
NC: Negative control; BK: Blank

Other figure/table from this article

Table 1 Enrichment of phages in biopanning
Fig.1 The results of the specific positive clones identified by phage-ELISA
Fig.2 Indirect phage-ELISA for positive phage clones
PC: Positive control; NC: Negative control; BK: Blank
Fig.3 PCR products for recombinant plasmid colony of A25、A26
M: DNA Maker; 1 ~ 4: A25 recombinant colonies; 5 ~ 8: A26 recombinant colonies
Fig.4 Comparison of the deduced amino acid sequences of nanobodies
Fig.5 SDS-PAGE analysis of purification of nanobody
M: Protein maker; 1: A25 nickel column flowthrough; 2: A26 nickel column flowthrough; 3~4: Nickel column purified protein
Fig.7 QDs-SA-labeled mouse SP2/0 imaging