China Biotechnology

Isolation and Characterization of an Efficient Strain of Human Intestinal Origin for Uric Acid Degradation

XU Tiancheng, CHEN Biao, CUI Xiaoyi, YE Li

China Biotechnology, 2025, 45(12): 1-13. DOI: 10.13523/j.cb.202506010

实验项目	结果	判断标准	生物学意义
靛基质试验	+	玫瑰红色环为阳性	分解色氨酸生成吲哚
甲基红试验	+	红色为阳性(pH≤4.4)	混合酸发酵产物降低培养基pH
二乙酰试验	-	无红色复合物为阴性	无乙酰甲基甲醇生成
柠檬酸盐利用	-	培养基保持绿色为阴性	无法利用柠檬酸盐作为唯一碳源

Table 1 IMVC biochemical identification results and biological significance of strain nf-5

Other figure/table from this article

Fig.1 Transparent circle of strain nf-5 cultured on a uric acid plate for 24 hours
Fig.2 Uric acid metabolism effect of reselected strain
Fig.3 Colony morphology of strain nf-5
Fig.4 Gram staining microscopy image
Fig.5 Phylogenetic tree based on 16S rRNA gene sequence
Table 2 nf-5 drug sensitivity test results
Fig.6 The impact of different sterilization methods on the concentration of uric acid in liquid medium
Fig.7 OD₆₀₀ values under different dissolved oxygen conditions
Fig.8 Effect of dissolved oxygen conditions on uric acid metabolism of strain nf-5
Fig. 9 Uric acid concentration-time curve under different dissolved oxygen conditions
Fig.10 OD₆₀₀-time curve under different dissolved oxygen conditions
Fig.11 OD₆₀₀ values of nf-5 under different nutritional conditions at 18 hours
Fig.12 Changes in uric acid concentration within 18 hours under different nutritional conditions
Fig.13 Changes in uric acid concentration within 24 hours under different glucose concentrations
Fig.14 OD₆₀₀-time curve under different glucose concentrations
Fig.15 Uric acid concentration-time curve under different glucose concentrations
Fig.16 The impact of osmotic pressure regulation on uric acid degradatio
Fig.17 Changes in uric acid concentration within 18 hours at different glycerol concentrations