Comparative Characterization of Two <em>Arthrospira</em> Strains Isolated from Full-scale Raceway Pond

doi:10.13523/j.cb.20170504

China Biotechnology

2017, Vol. 37

Issue (5): 28-37 DOI: 10.13523/j.cb.20170504

Comparative Characterization of Two Arthrospira Strains Isolated from Full-scale Raceway Pond

YAO Chang-hong¹, WU Pei-chun¹, CAO Xu-peng¹, LIU Jiao^1,2, JIANG Jun-peng^1,2, XUE Song¹

1. Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

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Abstract

Temperature is an important factor affecting biomass productivity in large-scale cultivation of Arthrospira. To improve Arthrospira production, it is crucial to screen strains with high productivity and excellent temperature-tolerance ability. Two strains Arthrospira sp. DICP-D (D) and Arthrospira sp. DICP-F (F) were isolated and identified from full-scale Arthrospira cultivation raceway pond. Although strain D and strain F displayed significant difference in morphology, they shared closest phylogenetic relationship among the existing Arthrospira strains with accessible 16S rRNA gene information. Under normal conditions, strain D and strain F harbor almost the same biomass compositions, and they also accumulated identical carbohydrate content in cell under nitrogen stress or low temperature stress conditions. However, the biomass productivity in strain D was 33% to 230% higher than that in strain F under the same cultivation conditions tested. Strain D showed 1.1 times better tolerance ability towards high temperature stress than strain F. The biomass productivity in strain D under 41℃ and 15℃ could be maintained at 73% and 61%, respectively, of that under normal temperature, demonstrating that strain D had good tolerance ability towards temperature stress. Strain D held more efficient photoprotection mechanism than strain F did, which endowed it better ability to acclimate to stressful conditions. The excellent biomass productivity and stress tolerance ability in strain D could make it applicable with great potential in outdoor large-scale Arthrospira cultivation.

Key words： Temperature Stress tolerance Algal strain selection Arthrospira

Received: 22 December 2016 Published: 25 May 2017

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YAO Chang-hong, WU Pei-chun, CAO Xu-peng, LIU Jiao, JIANG Jun-peng, XUE Song. Comparative Characterization of Two Arthrospira Strains Isolated from Full-scale Raceway Pond. China Biotechnology, 2017, 37(5): 28-37.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20170504 OR https://manu60.magtech.com.cn/biotech/Y2017/V37/I5/28

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