Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (8): 67-73    DOI: 10.13523/j.cb.20140811
    
Glycopeptides Extraction Using Hyperpolymer-assisted Hydrazide Functionalized Microsparticles
DENG Shan-shan1,2, WANG Ming-chao2, CAO Qi-chen2, BAI Hai-hong2, PEN Bo2, YING Wan-tao1,2, CAI Yun1,2
1. Graduate Department of Anhui Medical University, Hefei 230032, China;
2. State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China
Download: HTML   PDF(1133KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

Objective: A novel approach was developed to selective extraction of glycoproteins by hyperpolymer-assisted hydrazide functionalized microsparticles(HHMs). Methods: Firstly, synthesizing hyperbranched poly-glycerols (HPG) by ring-opening multibranching polymerization of glycidol using dioxane as an emulsifying agent following converted them to aldehyde-functional hyperbranched polyglycerol (HPG-ALD). Upon immobilization on the microspheres, aldehyde group of HPG-ALD were convert to hydrazide group via a reductive alkylation reaction. Conclusion: After characterized by scanning electron microscope (SEM) and Thermo Gravimetric Analysis (TGA), it can be confirmed that HHMs were successfully synthesized and effectively used for the low-abundance glycoproteins/glycopeptides enrichment.



Key wordsHHMs      Glycopeptide      Enrichment     
Received: 26 April 2014      Published: 25 August 2014
ZTFLH:  Q819  
Cite this article:

DENG Shan-shan, WANG Ming-chao, CAO Qi-chen, BAI Hai-hong, PEN Bo, YING Wan-tao, CAI Yun. Glycopeptides Extraction Using Hyperpolymer-assisted Hydrazide Functionalized Microsparticles. China Biotechnology, 2014, 34(8): 67-73.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140811     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I8/67


[1] Hgglund P, Bunkenborg J, Elortza F, et al. A new strategy for identification of N-glycosylated proteins and unambiguous assignment of their glycosylation sites using HILIC enrichment and partial deglycosylation. Proteome Res, 2004, 3(3): 556-566.

[2] Freeze, H H, Aebi M. Altered glycan structures: the molecular basis of congenital disorders of glycosylation. Current Opinion in Structural Biology, 2005, 15(5): 490-498.

[3] Leroy J G. Congenital disorders of N-glycosylation including diseases associated with O-as well as N-glycosylation defects. Pediatr Res, 2006, 60(6): 643-656.

[4] Slawson C, Hart G W. O-GlcNAc signalling: implications for cancer cell biology. Nat Rev Cancer, 2011, 11(9): 673-684.

[5] Hongqiang Qin, Liang Zhao, Ruibin Li, et al. Size-selective enrichment of N-linked glycans using highly ordered mesoporous carbon material and detection by MALDI-TOF MS. Anal Chem, 2011, 83(20): 7721-7728.

[6] Huang G, Xiong Z, Qin H, et al. Synthesis of zwitterionic polymer brushes hybrid silica nanoparticles via controlled polymerization for highly efficient enrichment of glycopeptides. Anal Chim Acta, 2014, 809(27): 61-68.

[7] Hengye Li, Heye Wang, Yun chun, et al. A benzoboroxole-functionalized monolithic column for the selective enrichment and separation of cis-diol containing biomolecules. Chemical Communications, 2012, 48(34): 4115-4117.

[8] Nishikaze T, Kawabata S, Iwamoto S, et al. Reversible hydrazide chemistry-based enrichment for O-GlcNAc-modified peptides and glycopeptides having non-reducing GlcNAc residues. Analyst, 2013, 138(23): 7224-7232.

[9] Jing Chen, Punit Shah, Hui Zhang, et al. Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical and Bioanalytical Chemistry, 2013, 5(22): 10670-10674.

[10] Zhong Tianping, Ai Pengfei, Zhou Jian, et al.Structures and properties of PAMAM dendrimer: a multi-scale simulation study. Fluid Phase Equilibria, 2011, 302(1): 43-47.

[11] Pan L, Iliuk A, Yu S, et al. Multiplexed quantitation of protein expression and phosphorylation based on functionalized soluble nanopolymers. J Am Chem Soc, 2012, 134(44): 18201-18204.

[12] Iliuk A B, Martin V A, Alicie B M, et al. In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion-functionalized soluble nanopolymers. Mol Cell Proteomics, 2010, 9(10): 2162-2172.

[13] Oded Kleifeld, Alain Doucet, Ulrich auf dem Keller, et al. Isotopic labeling of terminal amines in complex samples identifies protein N-termini and protease cleavage products. Nature Biotechnology, 2010, 28(3): 281-288.

[14] Cao Qichen, Ma C, Bai H, et al. Multivalent hydrazide-functionalized magnetic nanoparticles for glycopeptide enrichment and identification. Analyst, 2014, 139(3): 603-609.

[15] Zhang Ying, Kuang Min, Zhang Lijuan, et al. An accessible protocol for solid-phase extraction of N-linked glycopeptides through reductive amination by amine-functionalized magnetic nanoparticles. Anal Chem, 2013, 85(11): 5535-5541.

[16] Pan Yiting, Bai Haihong, Ma Cheng, et al. Brush polymer modified and lectin immobilized core-shell microparticle for highly efficient glycoprotein/glycopeptide enrichment. Talanta, 2013, 115: 842-848.

[17] Deng Shanshan, Cao Qichen, Ma Chen, et al. Amine-based soluble nanopolymers for highly effective glycopeptide enrichment. Letters in Biotechnology, 2013, 25: 158-164.

[18] Patrick Beaudette, Xifei Yu, Rajesh A, et al. Development of soluble ester-linked aldehyde polymers for proteomics. Anal Chem, 2011, 83(17): 6500-6510.

[19] Gao C, Yan D. Hyperbranched polymers: from synthesis to applications. Progress in Polymer Science, 2004, 29(3): 183-275.

[20] Hu M, Chen M, Li G. Biodegradable hyperbranched polyglycerol with ester linkages for drug delivery. Biomacromolecules, 2012, 13(11): 3552-3561.

[21] Carsten Gottschalk, Florian Wolf, Holger Frey. Multi-arm star poly(L-lactide) with hyperbranched polyglycerol core. Macromolecular Chemistry and Physics, 2007, 208(15): 1657-1665.

[22] Li Zhou, Chao Gao, Xu W, et al.Enhanced biocompatibility and biostability of CdTe quantum dots by facile surface-initiated dendritic polymerization. Biomacromolecules, 2009, 10(7): 1865-1874.

[1] MA Li-li,YI Pan-pan,AO Ni-hua,JIAO Hong-tao,LEI Rui-peng,LIU Huan. A Study of Interdisciplinarity in Biosafety Research Based on Discipline Categories and Enrichment Analysis[J]. China Biotechnology, 2021, 41(12): 116-124.
[2] Hong-yuan CHEN,Hong-yan CHEN,Chun QIAO,Jian-yong LI,Da-ru LU. The Establishment of a Novel Detection System for MYD88 L265P in Waldenström’s Macroglobulinemia[J]. China Biotechnology, 2018, 38(9): 35-40.
[3] Yi HUANG,Xiao-yu LI,Fang TIAN,Xiao-hong QIAN,Wan-tao YING. Identification and Quantitative Study on Glycosylation Chain Modification of Antibody Drugs by Mass Spectrometry[J]. China Biotechnology, 2018, 38(1): 32-41.
[4] DUAN Sheng-wen, LIU Zheng-chu, ZHENG Ke, FENG Xiang-yuan, CHENG Li-feng, ZHENG Xia. Technique for Exploring Pectinase Gene of Bast Fiber Degumming from Enrichment Liquid[J]. China Biotechnology, 2014, 34(1): 86-89.
[5] SHEN Jian, ZHANG Yue, PAN Qiu-hui, SUN Fen-yong. Bioinformatics Analysis and Prediction of miR-17-92 Cluster Mediated Regulatory Network[J]. China Biotechnology, 2012, 32(03): 69-75.
[6] ZOU Yang, XUE Meng-yang, ZHAO Ying-hua, CHEN Min. Endoglycosidase-based Chemoenzymatic Synthesis of Homogeneous Glycopeptides and Glycoproteins Carrying Defined N-Glycans[J]. China Biotechnology, 2012, 32(02): 107-116.