| Orginal Article |
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| The Establishment of a Novel Detection System for MYD88 L265P in Waldenström’s Macroglobulinemia |
Hong-yuan CHEN1,Hong-yan CHEN1,Chun QIAO2,Jian-yong LI2,Da-ru LU1,**( ) |
1 School of Life Science, Fudan University, Shanghai 200438, China
2 The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital,Key Laboratory of Hematology of Nanjing Medical University, Collaborative Innovation Center for Cancer Personalized Medicine,Nanjing 210029,China |
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Abstract Waldenström’s macroglobulinemia (WM) is a rare, incurable lymphoplasmacytic lymphoma (LPL). Research has showed that MYD88 L265P is commonly detected in patients with WM (>90%). Therefore this mutation could be used as a biomarker for disease diagnosis and differentiation. Current detection methods used at this mutation are of low sensitivity, which limit the clinical utility for early diagnosis. Thus, there is an urgent need for a more sensitive method to fulfill the requirement of early diagnosis. By combining ARMS and clamping PCR together, a novel detection system with higher sensitivity was developed. The limit of detection of the new system was 10 2 copies and the sensitivity was 0.1%. A double-blind trial with 19 clinical samples was conducted and the results completely accorded with the sample information. This new technique has a huge potential in clinical practices, especially early diagnosis, due to its increased accuracy and sensitivity.
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Received: 02 April 2018
Published: 12 October 2018
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Corresponding Authors:
Da-ru LU
E-mail: darulu@163.com
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