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| Comparison of Two Aspergillus niger Mutant and Wild Strains Based on q-rate and Flux Balance Analysis |
| CHEN Xiang-fen, LU Hong-zhong, TANG Wen-jun, TANG Yin, CHU Ju, ZHUANG Ying-pin, ZHANG Si-liang |
| State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China |
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Abstract Aspergillus niger is widely used in industrial enzyme production for its excellent protein expression and secretion capacity. The differences of physiological behaviors and metabolic flux distribution between Aspergillus niger mutant and wild strains under same cultivation conditions are investigated, so as to determine limited factors in glucoamylase production. Based on kinetic analysis, it is confirmed that the mutant strain gets a higher maximum specific growth rate (+30%), a lower by-product productivity (－90%) and a higher substrate uptake efficiency (+30%), which implies significant differences in carbon distribution and substrate usage efficiency between these two strains. By applying Flux Balance Analysis (FBA), it is found that supplies of reducing power and ribose are main factors which effect cell growth. What's more, precursor amino acids is confirmed to be the main limited factor in glucoamylase production. These conclusions provide significances for subsequent bioprocess optimization and strain gene modification.
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Received: 05 May 2014
Published: 25 August 2014
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