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| Effect of the Deletion of CcpA on Virulence in Listeria monocytogenes |
| JIANG Li-han, CHENG Xiao-long, QU Hui-ping, HUANG Yi-yi, WANG Shu-jia, LUO Qin |
| Central China Normal University, College of Life Science, Wuhan 430079, China |
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Abstract CcpA, encoded by the ccpA gene, is the global mediator of carbon catabolite repression (CCR) in gram-positive bacteria. Increasing evidences suggest that CcpA not only participate in CCR, but also regulate the expression of virulence genes in pathogenic bacteria directly or indirectly. To investigate the effect of CcpA on virulence of Listeria monocytogenes, CcpA deletion strain was constructed using homologous recombination. The LD50 of the wild strain EGDe and EGDeΔccpA for BLAB/c mice was measured, the amount of bacteria in liver and spleen was counted, and pathological changes of liver and spleen were observed. The results showed that compared to EGDe, the LD50 of EGDeΔccpA reduced by 10 times, and lesion caused by EGDeΔccpA in liver and spleen of mice was more severe, indicating that deletion of CcpA enhances bacterial virulence in L. monocytogenes, CcpA might regulate L. monocytogenes, virulence gene expression directly or indirectly.
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Received: 18 June 2014
Published: 25 August 2014
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[1] 吴艳, 顾阳, 任聪, 等. 微生物分解代谢物控制蛋白CcpA的研究进展. 生命科学, 2011, 23(9): 882-890. Wu Y, Gu Y, Ren C, et al. Recent research on catabolite control protein A in microorganisms. Chinese Bulletin of Life Sciences, 2011, 23(9): 882-890.
[2] Stülke J, Hillen W. Carbon catabolite repression in bacteria. Current Option in Microbiology, 1999, (2): 195-201.
[3] Mendez M, Huang I H, Ohtani K, et al. Carbon catabolite repression of type IV pilus-dependent gliding motility in the anaerobic pathogen Clostridium perfringens. Journal of Bacteriology, 2008, 190(1): 48-60.
[4] Willenborg J, Fulde M, de Greeff A, et al. Role of glucose and CcpA in capsule expression and virulence of Streptococcus suis. Microbiology, 2011, 157(6): 1823-1833.
[5] Seidl K, Stucki M, Ruegg M, et al. Staphylococcus aureus CcpA affects virulence determinant production and antibiotic resistance. Antimicrobial Agents and Chemotherapy, 2006, 50(4): 1183-1194.
[6] Antunes A, Martin-Verstraete I, Dupuy B. CcpA-mediated repression of Clostridium difficile toxin gene expression. Molecular Microbiology, 2011, 79(4): 882-899.
[7] Varga J, Stirewalt V L, Melville S B. The CcpA protein is necessary for efficient sporulation and enterotoxin gene (cpe) regulation in Clostridium perfringens. Journal of Bacteriology, 2004, 186(16): 5221-5229.
[8] Vazquez-Boland J A, Kuhn M, Berche P, et al. Listeria pathogenesis and molecular virulence determinants. Clinical Microbiology Reviews, 2001, 14(3): 584-640.
[9] Allerberger F, Wagner M. Listeriosis: a resurgent foodborne infection. Clinical Microbiology and Infection, 2010, 16(1): 16-23.
[10] Behari J, Youngman P. A homolog of CcpA mediates catabolite control in Listeria monocytogenes but not carbon source regulation of virulence genes. Journal of Bacteriology, 1998, 180(23): 6316-6324.
[11] 王莉, 冯飞飞, 张强, 等. 单核细胞增生李斯特菌毒力基因inlB/actA双缺失突变株的构建. 生物技术通报, 2010, 11: 182-185. Wang L, Feng F F, Zhang Q, et al. Construction of a mutant strain of Listeria monocytogenes with a deletion of inlB and actA. Biotechnology Bulletin, 2010, 11: 182-185.
[12] 张再超, 孟庆玲, 乔军, 等. RsbV基因缺失对单核细胞增生李斯特菌毒力的影响. 微生物学报, 2013, 53(1): 59-65. Zhang Z C, Meng Q L, Qiao J, et al. Effects of the deletion of RsbV gene on virulence of Listeria monocytogenes Acta Microbiologica Sinica, 2013, 53(1): 59-65.
[13] 殷月兰, 张晨菊, 田德斌, 等. 产单核细胞李氏杆菌突变株的感染动力学研究. 中国兽医科学, 2007, 37(10): 850-853. Yin Y L, Zhang C J, Tian D B, et al. Kinetics of mutant Listeria monocytogenes strain yzuLM122 in murine infection model. Veterinary Science in China, 2007, 37(10): 850-853.
[14] Dussurget O, Pizarro-Cerda J, Cossart P. Molecular determinants of Listeria monocytogenes virulence. Annual Review of Microbiology, 2004, 58: 587-610.
[15] Tsuchiya K, Kawamura I, Takahashi A, et al. Listeriolysin O-induced membrane permeation mediates persistent interleukin-6 production in Caco-2 cells during Listeria monocytogenes infection in vitro. Infection and Immunity, 2005, 73 (7): 3869-3877.
[16] Pentecost M, Otto G, Theriot J A, et al. Listeria monocytogenes invades the epithelial junctions at sites of cell extrusion. PLoS Pathogens, 2006, 2(1): e3.
[17] Kinkel T L, Mclver K S. CcpA-mediated repression of streptolysin S expression and virulence in the group A streptococcus. Infection and Immunity, 2008, 76(8): 3451-3463.
[18] 罗勤, 张晓莉, 李兵, 等. 单核细胞增生李斯特菌PrfA蛋白转录调控毒力基因表达的分子机制. 微生物学通报, 2008, 35(2): 275-280. Luo Q, Zhang X L, Li B, et al. Regulation of PrfA-dependent virulence genes expression in Listeria monocytogenes. Microbiology, 2008, 35(2): 275-280.
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