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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (1): 38-46    DOI: 10.13523/j.cb.20160106
    
De novo Characterization of the Seed Transcriptome of Lepidium apetalum Willd
ZHOU Qian1, ZHAO Hui-xin1, LI Ping-ping1, ZENG Wei-jun1, LI Yan-hong1, GE Feng-wei1, ZHAO Jun-jie1, ZHAO He-ping2
1. Xinjiang Key Laboratory of Special Species Diversity Application and Regulatory, College of Life Science, Xinjiang Normal University, Urumqi 830054, China;
2. Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Science, Beijing Normal University, Beijing 100875, China
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Abstract  

Lepidium apetalum Willd is an important traditional Chinese medicine. Various active components have been extracted from the Lepidium apetalum. However, the genetic basis for their activity is virtually unknown. The transcriptome of Lepidium apetalum was sequenced using the Illumina HiSeqTM 2000 sequencing platform. The clean reads were then de novo assembled into 40 303 unigenes. 27 935 unigene were annotated by a similarity search against SiX public databases. The results showed that 534 genes were assigned to second metabolic pathway. Among them, 4 unigenes were mapped to the glucosinolate, 19 to flavonoids, stilbenoid, diarylheptanoid, 69 to gingerol shikimate biosynthesis pathways, and 92 unigenes were respectively mapped to the phenylalanine metabolism pathways, suggesting that they are involved in these pathways of pharmaceutically important. Thirteen homologous fragments of key genes identified were referred to these pathways. In addition, a total of 6 304 SSRs were identified from the sequence of transcription, distributed in 5 306 unigenes(15.64%). This work not only provides many valuable basal data for gene cloning and molecular biology research, but also lays the foundation for genetic diversity analysis and development of molecular marker in Lepidium apetalum.

Key words： Second metabolism      Transcriptome      Lepidium apetalum Willd      Simple sequence repeat     
Received: 08 September 2015      Published: 11 January 2016
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ZHOU Qian
LI Ping-Ping
ZENG Wei-Jun
LI Yan-Hong
GE Feng-Wei
YANG Na
DIAO Hui-Xin
Cite this article:

ZHOU Qian, ZHAO Hui-xin, LI Ping-ping, ZENG Wei-jun, LI Yan-hong, GE Feng-wei, ZHAO Jun-jie, ZHAO He-ping. De novo Characterization of the Seed Transcriptome of Lepidium apetalum Willd. China Biotechnology, 2016, 36(1): 38-46.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160106     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I1/38

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