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| Engineering of an Escherichia coli Strain LHY02 for Production of Optically Pure D-lactic Acid from Xylose |
| LU Hong-ying, HE Hu, LIU Zao, WANG Yong-ze, WANG Jin-hua |
| Hubei Cooperative Innovation Center for Industrial Fermentation, Key Laboratory of Fermentation Engineering Ministry of Education, Hubei University of Technology, Wuhan 430068, China |
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Abstract Efficient utilization of xylose is an import microbial trait for fermentative production of bio-based products using renewable lignocellulosic feedstocks. Escherichia coli WL204 was previously engineered for fermentative production of optically pure L-lactic acid from xylose. E. coli WL204 was reengineered for D-lactic acid production by functionally replacing the l-lactate dehydrogenase gene (ldhL) with a D-lactate dehydrogenase (ldhA), resulting in strain E. coli LHY02. In 10% xylose fermentation test, LHY02 produced 84 g/L D-lactic acid with an optical purity of 99.5% and a volumetric productivity of 0.93 g/L/h.
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Received: 08 October 2014
Published: 25 December 2014
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